Genetic recombination is used as a tool for modifying the composition of poxvirus genomes
in both discovery and applied research. This review documents the history behind the …

Nanodrug delivery systems have demonstrated a great potential for tumor therapy with the
development of nanotechnology. Nonetheless, traditional drug delivery systems are faced …

The Redβ protein of the bacteriophage λ red recombination system is a model annealase
which catalyzes single-strand annealing homologous DNA recombination. Here we present …

Highlights•Phage therapy is emerging as an alternative to antibiotics.•The first case of phage
therapy in humans using engineered phages is discussed.•This was also the first case of …

Recombineering assisted multiplex genome editing generally uses single-stranded
oligonucleotides for site directed mutational changes. It has proven highly efficient for …

Porphyromonas gingivalis is a major oral bacterial pathogen responsible for severe
periodontal diseases. Numerous studies have used genetic approaches to elucidate the …

Recombineering is an essential tool for molecular biologists, allowing for the facile and
efficient manipulation of bacterial genomes directly in cells without the need for costly and …

There is an increasing demand for bioproducts produced by metabolically engineered
microbes, such as pharmaceuticals, biofuels, biochemicals and other high value …

Bacteriophages—viruses that infect bacterial cells—are the most abundant biological
entities on Earth. The use of phages in fundamental research and industry requires tools for …

Herpesviruses are ubiquitous human pathogens, which are the causative agent of mild to
severe symptoms ranging from cold sore to nasopharyngeal carcinoma. Even though …