Uncovering the cis-regulatory code that governs when and how much each gene is
transcribed in a given genome and cellular state remains a central goal of biology. Here, we …

The Mediator complex, which in humans is 1.4 MDa in size and includes 26 subunits,
controls many aspects of RNA polymerase II (Pol II) function. Apart from its size, a defining …

Components of transcriptional machinery are selectively partitioned into specific
condensates, often mediated by protein disorder, yet we know little about how this specificity …

Regulation of biological processes typically incorporates mechanisms that initiate and
terminate the process and, where understood, these mechanisms often involve feedback …

Genomes have complex three-dimensional architectures. The recent convergence of
genetic, biochemical, biophysical, and cell biological methods has uncovered several …

We perform a thorough analysis of RNA velocity methods, with a view towards
understanding the suitability of the various assumptions underlying popular …

In eukaryotic cells, protein and RNA factors involved in genome activities like transcription,
RNA processing, DNA replication, and repair accumulate in self-organizing membraneless …

Transcription factors (TFs) consist of a DNA-binding domain and an activation domain (AD)
that are frequently considered to be independent and exchangeable modules. However …

The Polycomb system has fundamental roles in regulating gene expression during
mammalian development. However, how it controls transcription to enable gene repression …

Cells must tightly regulate their gene expression programs and yet rapidly respond to acute
biochemical and biophysical cues within their environment. This information is transmitted to …