CRISPR-based diagnostics
MM Kaminski, OO Abudayyeh, JS Gootenberg… - Nature Biomedical …, 2021 - nature.com
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …
Point-of-care diagnostics for infectious diseases: From methods to devices
C Wang, M Liu, Z Wang, S Li, Y Deng, N He - Nano Today, 2021 - Elsevier
The current widespread of COVID-19 all over the world, which is caused by SARS-CoV-2
virus, has again emphasized the importance of development of point-of-care (POC) …
virus, has again emphasized the importance of development of point-of-care (POC) …
Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay
The recent outbreak of novel coronavirus (SARS-CoV-2) causing COVID-19 disease
spreads rapidly in the world. Rapid and early detection of SARS-CoV-2 facilitates early …
spreads rapidly in the world. Rapid and early detection of SARS-CoV-2 facilitates early …
CRISPR-Cas12a-based nucleic acid amplification-free DNA biosensor via Au nanoparticle-assisted metal-enhanced fluorescence and colorimetric analysis
Cell-free DNA (cfDNA) has attracted significant attention due to its high potential to diagnose
diseases, such as cancer. Still, its detection by amplification method has limitations because …
diseases, such as cancer. Still, its detection by amplification method has limitations because …
A highly sensitive CRISPR‐empowered surface plasmon resonance sensor for diagnosis of inherited diseases with femtomolar‐level real‐time quantification
The clustered regularly interspaced short palindromic repeats (CRISPR) molecular system
has emerged as a promising technology for the detection of nucleic acids. Herein, the …
has emerged as a promising technology for the detection of nucleic acids. Herein, the …
Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities
JE van Dongen, JTW Berendsen… - Biosensors and …, 2020 - Elsevier
With the trend of moving molecular tests from clinical laboratories to on-site testing, there is a
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …
Recent progress in non-native nucleic acid modifications
While Nature harnesses RNA and DNA to store, read and write genetic information, the
inherent programmability, synthetic accessibility and wide functionality of these nucleic acids …
inherent programmability, synthetic accessibility and wide functionality of these nucleic acids …
Advances in nucleic acid amplification techniques (NAATs): COVID-19 point-of-care diagnostics as an example
Achieving superhigh sensitivity is the ultimate goal for bio-detection in modern analytical
science and life science. Among variable signal amplification strategies, nucleic acid …
science and life science. Among variable signal amplification strategies, nucleic acid …
The CRISPR–Cas toolbox for analytical and diagnostic assay development
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
associated (Cas) systems have revolutionized biological and biomedical sciences in many …