The development and application of emerging technologies of Industry 4.0 enable the
realization of digital twins (DT), which facilitates the transformation of the manufacturing …

Typical bioprocess comprises of different unit operations wherein a near optimal
environment is required for cells to grow, divide, and synthesize the desired product …

Among the greatest challenges in soft sensor development for bioprocesses are variable
process lengths, multiple process phases, and erroneous model inputs due to sensor faults …

Continuous bioprocessing is significantly changing the biological drugs (or biologics)
manufacturing landscape by potentially improving product quality, process stability, and …

In this study, we demonstrated the first, to our knowledge, integrated continuous bioprocess
(ICB) designed for the production of acid‐sensitive monoclonal antibodies, prone to …

Highlights•Growing application of continuous processing in manufacturing protein
therapeutics.•Developing continuous processing for viral vector, vaccine, and cell therapy …

The N-linked glycosylation pattern is an important quality attribute of therapeutic
glycoproteins. It has been reported by our group and by others that different carbon sources …

A novel approach of design of experiment (DoE) is developed for the optimization of key
substrates of the culture medium, amino acids, and sugars, by utilizing perfusion …

The economic benefits of continuous PEG precipitation of antibodies compared to
continuous capture by protein A affinity chromatography have been always lowered by the …

The safety requirements for vaccines are extremely high since they are administered to
healthy people. For that reason, vaccine development is time-consuming and very …