Microbial source tracking markers for detection of fecal contamination in environmental waters: relationships between pathogens and human health outcomes

VJ Harwood, C Staley, BD Badgley… - FEMS microbiology …, 2014 - academic.oup.com
Microbial source tracking (MST) describes a suite of methods and an investigative strategy
for determination of fecal pollution sources in environmental waters that rely on the …

Microbial indicators of fecal pollution: recent progress and challenges in assessing water quality

DA Holcomb, JR Stewart - Current environmental health reports, 2020 - Springer
Abstract Purpose of Review Fecal contamination of water is a major public health concern.
This review summarizes recent developments and advancements in water quality indicators …

Reporting the limits of detection and quantification for environmental DNA assays

KE Klymus, CM Merkes, MJ Allison… - Environmental …, 2020 - Wiley Online Library
Abstract Background Environmental DNA (eDNA) analysis is increasingly being used to
detect the presence and relative abundance of rare species, especially invasive or imperiled …

Droplet digital PCR for simultaneous quantification of general and human-associated fecal indicators for water quality assessment

Y Cao, MR Raith, JF Griffith - water research, 2015 - Elsevier
Despite wide application to beach water monitoring and microbial source identification,
results produced by quantitative PCR (qPCR) methods are subject to bias introduced by …

Human fecal contamination of water, soil, and surfaces in households sharing poor-quality sanitation facilities in Maputo, Mozambique

DA Holcomb, J Knee, T Sumner, Z Adriano… - International Journal of …, 2020 - Elsevier
Identifying the origin of fecal contamination can support more effective interventions to
interrupt enteric pathogen transmission. Microbial source tracking (MST) assays may help to …

[HTML][HTML] Integrating culture-based and molecular methods provides an improved assessment of microbial quality in a coastal lagoon

M Basili, L Perini, L Zaggia, GM Luna, GM Quero - Environmental Pollution, 2023 - Elsevier
Faecal pollution in aquatic environments is a worldwide public health concern, yet the
reliability and comprehensiveness of the methods used to assess faecal contamination are …

Performance of human fecal anaerobe-associated PCR-based assays in a multi-laboratory method evaluation study

BA Layton, Y Cao, DL Ebentier, K Hanley, E Balleste… - Water Research, 2013 - Elsevier
A number of PCR-based methods for detecting human fecal material in environmental
waters have been developed over the past decade, but these methods have rarely received …

Source tracking swine fecal waste in surface water proximal to swine concentrated animal feeding operations

CD Heaney, K Myers, S Wing, D Hall, D Baron… - Science of the total …, 2015 - Elsevier
Swine farming has gone through many changes in the last few decades, resulting in
operations with a high animal density known as confined animal feeding operations …

Isolating the impact of septic systems on fecal pollution in streams of suburban watersheds in Georgia, United States

RA Sowah, MY Habteselassie, DE Radcliffe, E Bauske… - Water research, 2017 - Elsevier
The presence of multiple sources of fecal pollution at the watershed level presents
challenges to efforts aimed at identifying the influence of septic systems. In this study …

Data acceptance criteria for standardized human-associated fecal source identification quantitative real-time PCR methods

OC Shanks, CA Kelty, R Oshiro… - Applied and …, 2016 - Am Soc Microbiol
There is growing interest in the application of human-associated fecal source identification
quantitative real-time PCR (qPCR) technologies for water quality management. The …