Rotavirus research: 2014–2020

S Caddy, G Papa, A Borodavka, U Desselberger - Virus research, 2021 - Elsevier
Rotaviruses are major causes of acute gastroenteritis in infants and young children
worldwide and also cause disease in the young of many other mammalian and of avian …

Coelenterazine-dependent luciferases as a powerful analytical tool for research and biomedical applications

VV Krasitskaya, EE Bashmakova, LA Frank - International Journal of …, 2020 - mdpi.com
The functioning of bioluminescent systems in most of the known marine organisms is based
on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by …

Mucosal and systemic neutralizing antibodies to norovirus induced in infant mice orally inoculated with recombinant rotaviruses

T Kawagishi, L Sánchez-Tacuba… - Proceedings of the …, 2023 - National Acad Sciences
Rotaviruses (RVs) preferentially replicate in the small intestine and frequently cause severe
diarrheal disease, and the following enteric infection generally induces variable levels of …

N-glycosylation of rotavirus NSP4 protein affects viral replication and pathogenesis

JA Nurdin, T Kotaki, T Kawagishi, S Sato… - Journal of …, 2023 - Am Soc Microbiol
Rotavirus (RV), the most common cause of gastroenteritis in children, carries a high
economic and health burden worldwide. RV encodes six structural proteins and six …

Generation of recombinant rotavirus expressing NSP3-UnaG fusion protein by a simplified reverse genetics system

AA Philip, JL Perry, HE Eaton, M Shmulevitz… - Journal of …, 2019 - Am Soc Microbiol
Rotavirus is a segmented double-stranded RNA (dsRNA) virus that causes severe
gastroenteritis in young children. We have established an efficient simplified rotavirus …

[PDF][PDF] On the stability of sequences inserted into viral genomes

A Willemsen, MP Zwart - Virus Evolution, 2019 - academic.oup.com
Viruses are widely used as vectors for heterologous gene expression in cultured cells or
natural hosts, and therefore a large number of viruses with exogenous sequences inserted …

Reverse genetics system for a human group A rotavirus

T Kawagishi, JA Nurdin, M Onishi, R Nouda… - Journal of …, 2020 - Am Soc Microbiol
Group A rotavirus (RV) is a major cause of acute gastroenteritis in infants and young
children worldwide. Recently, we established an entirely plasmid-based reverse genetics …

Reverse genetics approach for developing rotavirus vaccine candidates carrying VP4 and VP7 genes cloned from clinical isolates of human rotavirus

Y Kanai, M Onishi, T Kawagishi, P Pannacha… - Journal of …, 2020 - Am Soc Microbiol
Species A rotaviruses (RVs) are a leading cause of severe acute gastroenteritis in infants
and children younger than 5 years. Currently available RV vaccines were adapted from wild …

Recent advances in rotavirus reverse genetics and its utilization in basic research and vaccine development

T Uprety, D Wang, F Li - Archives of virology, 2021 - Springer
Rotaviruses are segmented double-stranded RNA viruses with a high frequency of gene
reassortment, and they are a leading cause of global diarrheal deaths in children less than 5 …

Production of OSU G5P [7] Porcine Rotavirus Expressing a Fluorescent Reporter via Reverse Genetics

AJ Snyder, CA Agbemabiese, JT Patton - Viruses, 2024 - mdpi.com
Rotaviruses are a significant cause of severe, potentially life-threatening gastroenteritis in
infants and the young of many economically important animals. Although vaccines against …