Optical higher harmonic generation, including second harmonic generation and third
harmonic generation, leaves no energy deposition to its interacted matters due to an energy …

Morphological changes and complex developmental processes inside vertebrate embryos
are difficult to observe noninvasively with millimeter-penetration and sub-micrometer …

Multi‐color whole‐mount in situ hybridization is a powerful technique for comparing the
spatial expression patterns of two or more genes in developing embryos. We have …

Nervous system development is a complicated dynamic process, and many mechanisms
remain unknown. By utilizing endogenous second-harmonic-generation as the contrast of …

Biophotonics is a new discipline in biophysics and has emerged from research and
development at the interface between the photonics and biomedical science and …

Three-dimensional computer reconstructions of gene expression data will become a
valuable tool in biomedical research in the near future. However, at present the process of …

Gene expression plays an important role in embryo development and organ function.
Previous studies have shown that harmonic generation microscopy (HGM) can be used as a …

We establish the advantages of two-color excitation (2CE) fluorescence microscopy where
the two confocal excitation beams are separated by an angle, θ [Opt. Lett. 24 (1999) 1505] …

We study the performance of two-color excitation (2CE) fluorescence microscopy [Opt. Lett.
24, 1505 (1999)] in turbid media of different densities and anisotropy. Excitation is achieved …

Abstract Via the Richards-Wolf vector diffraction theory, we analyze the three-dimensional
intensity distribution of the focal volume that is produced by a strongly focused 750-nm beam …