Structural biology of CRISPR–Cas immunity and genome editing enzymes
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …
a wide range of genome editing and biotechnological applications. In this Review, we …
The biology of CRISPR-Cas: backward and forward
F Hille, H Richter, SP Wong, M Bratovič, S Ressel… - Cell, 2018 - cell.com
In bacteria and archaea, clustered regularly interspaced short palindromic repeats
(CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system …
(CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system …
Phage anti-CBASS and anti-Pycsar nucleases subvert bacterial immunity
The cyclic oligonucleotide-based antiphage signalling system (CBASS) and the pyrimidine
cyclase system for antiphage resistance (Pycsar) are antiphage defence systems in diverse …
cyclase system for antiphage resistance (Pycsar) are antiphage defence systems in diverse …
Controlling and enhancing CRISPR systems
Many bacterial and archaeal organisms use clustered regularly interspaced short
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
Cryo-EM in drug discovery: achievements, limitations and prospects
JP Renaud, A Chari, C Ciferri, W Liu… - Nature reviews Drug …, 2018 - nature.com
Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …
technique that can be used to determine the structure of biological macromolecules and …
Anti-CRISPR: discovery, mechanism and function
CRISPR–Cas adaptive immune systems are widespread among bacteria and archaea.
Recent studies have shown that these systems have minimal long-term evolutionary effects …
Recent studies have shown that these systems have minimal long-term evolutionary effects …
Bacteriophages suppress CRISPR–Cas immunity using RNA-based anti-CRISPRs
Many bacteria use CRISPR–Cas systems to combat mobile genetic elements, such as
bacteriophages and plasmids. In turn, these invasive elements have evolved anti-CRISPR …
bacteriophages and plasmids. In turn, these invasive elements have evolved anti-CRISPR …
Antimicrobial activities of 1-phenyl-3-methyl-4-trichloroacetyl-pyrazolone: Experimental, DFT studies, and molecular docking investigation
The rate of bacterial resistance to antibiotics is faster than the rate of discovery of new
antibiotic classes. However, development of novel compounds with similar behavior but with …
antibiotic classes. However, development of novel compounds with similar behavior but with …
Disabling Cas9 by an anti-CRISPR DNA mimic
CRISPR (clustered regularly interspaced short palindromic repeats)–Cas9 gene editing
technology is derived from a microbial adaptive immune system, where bacteriophages are …
technology is derived from a microbial adaptive immune system, where bacteriophages are …
PAM identification by CRISPR-Cas effector complexes: diversified mechanisms and structures
D Gleditzsch, P Pausch, H Müller-Esparza, A Özcan… - RNA biology, 2019 - Taylor & Francis
Adaptive immunity of prokaryotes is mediated by CRISPR-Cas systems that employ a large
variety of Cas protein effectors to identify and destroy foreign genetic material. The different …
variety of Cas protein effectors to identify and destroy foreign genetic material. The different …