Structural basis of proline-specific exopeptidase activity as observed in human dipeptidyl peptidase-IV

R Thoma, B Löffler, M Stihle, W Huber, A Ruf, M Hennig - Structure, 2003 - cell.com
Inhibition of dipeptidyl peptidase IV (DPP-IV), the main glucagon-like peptide 1 (GLP1)-
degrading enzyme, has been proposed for the treatment of type II diabetes. We expressed …

Variation in N-Linked Oligosaccharide Structures on Heterologous Proteins Secreted by the Methylotrophic YeastPichia pastoris

R Montesino, R Garcıa, O Quintero… - Protein expression and …, 1998 - Elsevier
We report the characterization of N-linked oligosaccharides on six foreign glycoproteins
secreted from the methylotrophic yeastPichia pastoris. These proteins included: a bacterial …

Crystal structure of an ephrin ectodomain

J Toth, T Cutforth, AD Gelinas, KA Bethoney, J Bard… - Developmental cell, 2001 - cell.com
Eph receptor tyrosine kinases and their membrane-associated ligands, the ephrins, are
essential regulators of axon guidance, cell migration, segmentation, and angiogenesis …

Isotopic Labeling of Recombinant Proteins from the Methylotrophic Yeast Pichia pastoris

AR Pickford, JM O'Leary - Protein NMR techniques, 2004 - Springer
The methylotrophic yeast Pichia pastoris is now an established expression system for the
production of recombinant protein for nuclear magnetic resonance (NMR) studies. It is …

High-performance liquid chromatography in clinical analysis

DJ Anderson - Analytical chemistry, 1999 - ACS Publications
Applications of high-performance liquid chromatography (HPLC) in clinical analysis are
critically reviewed in the present article for the Chemical Abstracts time period from October …

Expression of Na+, K+-ATPase in Pichia pastoris: analysis of wild type and D369N mutant proteins by Fe2+-catalyzed oxidative cleavage and molecular modeling

D Strugatsky, KE Gottschalk, R Goldshleger… - Journal of Biological …, 2003 - ASBMB
Na+, K+-ATPase (pig α1, β1) has been expressed in the methylotrophic yeast Pichia
pastoris. A protease-deficient strain was used, recombinant clones were screened for …

Expression of a cold-adapted fish trypsin in Pichia pastoris

M Macouzet, BK Simpson, BH Lee - FEMS yeast research, 2005 - academic.oup.com
Trypsin is a highly valuable protease that has many industrial and biomedical applications.
The growing demand for non-animal sources of the enzyme and for trypsins with special …

High-level expression and production of human lactoferrin in

T Jiang, L Chen, S Jia, L Chen, Y Ma - Dairy Science and …, 2008 - dairy-journal.org
Lactoferrin (LF) is a multifunctional iron-binding glycoprotein which is found in high
concentrations in milk. Recombinant human LF (rhLF) may provide health benefits. In the …

Reaction of pyranose dehydrogenase from Agaricus meleagris with its carbohydrate substrates

MMH Graf, J Sucharitakul, U Bren, DB Chu… - The FEBS …, 2015 - Wiley Online Library
Monomeric Agaricus meleagris pyranose dehydrogenase (Am PDH) belongs to the glucose–
methanol–choline family of oxidoreductases. An FAD cofactor is covalently tethered to …

Effect of monosaccharide composition, glycosidic linkage position and anomericity on the electrophoretic mobility of labeled oligosaccharides

J Masuoka, KC Hazen - Electrophoresis, 2006 - Wiley Online Library
Fluorophore‐assisted carbohydrate electrophoresis (FACE) is useful for separation and
characterization of oligosaccharides from various sources and for comparing several …