The tracrRNA in CRISPR biology and technologies

C Liao, CL Beisel - Annual review of genetics, 2021 - annualreviews.org
CRISPR-Cas adaptive immune systems in bacteria and archaea utilize short CRISPR RNAs
(crRNAs) to guide sequence-specific recognition and clearance of foreign genetic material …

Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9

C Jiao, S Sharma, G Dugar, NL Peeck, T Bischler… - Science, 2021 - science.org
CRISPR-Cas systems recognize foreign genetic material using CRISPR RNAs (crRNAs). In
type II systems, a trans-activating crRNA (tracrRNA) hybridizes to crRNAs to drive their …

SgRNA engineering for improved genome editing and expanded functional assays

C Dong, Y Gou, J Lian - Current Opinion in Biotechnology, 2022 - Elsevier
Highlights•SgRNA engineering enables more powerful and versatile CRISPR/Cas9
systems.•The sgRNA engineering strategies are summarized in a structural …

Conformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells

PD Donohoue, M Pacesa, E Lau, B Vidal, MJ Irby… - Molecular Cell, 2021 - cell.com
The off-target activity of the CRISPR-associated nuclease Cas9 is a potential concern for
therapeutic genome editing applications. Although high-fidelity Cas9 variants have been …

Molecular mechanisms, off‐target activities, and clinical potentials of genome editing systems

N Zheng, L Li, X Wang - Clinical and Translational Medicine, 2020 - Wiley Online Library
Methodologies of genome editing are rapidly developing with the improvement of gene
science and technology, mechanism‐based understanding, and urgent needs. In addition to …

Natural Nucleoside Modifications in Guide RNAs Can Modulate the Activity of the CRISPR-Cas9 System In Vitro

DV Prokhorova, IP Vokhtantsev, PO Tolstova… - The CRISPR …, 2022 - liebertpub.com
At the present time, the clustered regularly interspaced short palindromic repeats (CRISPR)-
CRISPR-associated protein 9 (Cas9) system has been widely adopted as an efficient …

[HTML][HTML] Updates on CRISPR-based gene editing in HIV-1/AIDS therapy

Z Zhang, W Hou, S Chen - Virologica Sinica, 2022 - Elsevier
Although tremendous efforts have been made to prevent and treat HIV-1 infection, HIV-
1/AIDS remains a major threat to global human health. The combination antiretroviral …

A short overview of CRISPR-Cas technology and its application in viral disease control

A Ghorbani, S Hadifar, R Salari, K Izadpanah… - Transgenic …, 2021 - Springer
Abstract Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) together
with CRISPR-associated (Cas) proteins have catalysed a revolution in genetic engineering …

Conditional chemoconnectomics (cCCTomics) as a strategy for efficient and conditional targeting of chemical transmission

R Mao, J Yu, B Deng, X Dai, Y Du, S Du, W Zhang… - Elife, 2024 - elifesciences.org
Dissection of neural circuitry underlying behaviors is a central theme in neurobiology. We
have previously proposed the concept of chemoconnectome (CCT) to cover the entire …

Highly efficient neuronal gene knockout in vivo by CRISPR-Cas9 via neonatal intracerebroventricular injection of AAV in mice

S Hana, M Peterson, H McLaughlin, E Marshall… - Gene therapy, 2021 - nature.com
CRISPR-Cas systems have emerged as a powerful tool to generate genetic models for
studying normal and diseased central nervous system (CNS). Targeted gene disruption at …