CRISPR-Cas systems recognize foreign genetic material using CRISPR RNAs (crRNAs). In type II systems, a trans-activating crRNA (tracrRNA) hybridizes to crRNAs to drive their …
C Dong, Y Gou, J Lian - Current Opinion in Biotechnology, 2022 - Elsevier
Highlights•SgRNA engineering enables more powerful and versatile CRISPR/Cas9 systems.•The sgRNA engineering strategies are summarized in a structural …
PD Donohoue, M Pacesa, E Lau, B Vidal, MJ Irby… - Molecular Cell, 2021 - cell.com
The off-target activity of the CRISPR-associated nuclease Cas9 is a potential concern for therapeutic genome editing applications. Although high-fidelity Cas9 variants have been …
N Zheng, L Li, X Wang - Clinical and Translational Medicine, 2020 - Wiley Online Library
Methodologies of genome editing are rapidly developing with the improvement of gene science and technology, mechanism‐based understanding, and urgent needs. In addition to …
DV Prokhorova, IP Vokhtantsev, PO Tolstova… - The CRISPR …, 2022 - liebertpub.com
At the present time, the clustered regularly interspaced short palindromic repeats (CRISPR)- CRISPR-associated protein 9 (Cas9) system has been widely adopted as an efficient …
Z Zhang, W Hou, S Chen - Virologica Sinica, 2022 - Elsevier
Although tremendous efforts have been made to prevent and treat HIV-1 infection, HIV- 1/AIDS remains a major threat to global human health. The combination antiretroviral …
Abstract Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) together with CRISPR-associated (Cas) proteins have catalysed a revolution in genetic engineering …
R Mao, J Yu, B Deng, X Dai, Y Du, S Du, W Zhang… - Elife, 2024 - elifesciences.org
Dissection of neural circuitry underlying behaviors is a central theme in neurobiology. We have previously proposed the concept of chemoconnectome (CCT) to cover the entire …
S Hana, M Peterson, H McLaughlin, E Marshall… - Gene therapy, 2021 - nature.com
CRISPR-Cas systems have emerged as a powerful tool to generate genetic models for studying normal and diseased central nervous system (CNS). Targeted gene disruption at …