[HTML][HTML] Lipopeptides development in cosmetics and pharmaceutical applications: a comprehensive review

M Pilz, P Cavelius, F Qoura, D Awad, T Brück - Biotechnology Advances, 2023 - Elsevier
Lipopeptides are surface active, natural products of bacteria, fungi and green-blue algae
origin, having diverse structures and functionalities. In analogy, a number of chemical …

Forced degradation studies of biopharmaceuticals: Selection of stress conditions

E Tamizi, A Jouyban - European Journal of Pharmaceutics and …, 2016 - Elsevier
Stability studies under stress conditions or forced degradation studies play an important role
in different phases of development and production of biopharmaceuticals and biological …

Purification of modified therapeutic proteins available on the market: An analysis of chromatography-based strategies

C Sánchez-Trasviña, M Flores-Gatica… - … in Bioengineering and …, 2021 - frontiersin.org
Proteins, which have inherent biorecognition properties, have long been used as
therapeutic agents for the treatment of a wide variety of clinical indications. Protein …

Free radicals and ros induce protein denaturation by uv photostability assay

P Ruzza, C Honisch, R Hussain, G Siligardi - International Journal of …, 2021 - mdpi.com
Oxidative stress, photo-oxidation, and photosensitizers are activated by UV irradiation and
are affecting the photo-stability of proteins. Understanding the mechanisms that govern …

Lipidated short analogue of α-Melanocyte stimulating hormone exerts bactericidal activity against the stationary phase of methicillin-resistant staphylococcus aureus …

S Mumtaz, S Behera, K Mukhopadhyay - ACS omega, 2020 - ACS Publications
Stationary phase Staphylococcus aureus, especially methicillin-resistant S. aureus (MRSA),
has been widely associated with many persistent infections as well as biofilm-associated …

[HTML][HTML] Gold nanoparticles increases UV and thermal stability of human serum albumin

R Capomaccio, I Osório, I Ojea-Jiménez, G Ceccone… - …, 2016 - pubs.aip.org
Ultraviolet (UV) radiation, temperature, and time can degrade proteins. Here, the authors
show that gold nanoparticles significantly protect human serum albumin from denaturation …

UV-denaturation assay to assess protein photostability and ligand-binding interactions using the high photon flux of diamond B23 beamline for SRCD

R Hussain, E Longo, G Siligardi - Molecules, 2018 - mdpi.com
Light irradiation with high photon flux in the vacuum and far-UV region is known to denature
the conformation of biopolymers. Measures are in place at Diamond Light Source B23 …

Characterisation of conformational and ligand binding properties of membrane proteins using synchrotron radiation circular dichroism (SRCD)

R Hussain, G Siligardi - The Next Generation in Membrane Protein …, 2016 - Springer
Membrane proteins are notoriously difficult to crystallise for use in X-ray crystallographic
structural determination, or too complex for NMR structural studies. Circular dichroism (CD) …

Innovative chemical synthesis and conformational hints on the lipopeptide liraglutide

I Guryanov, A Bondesan, D Visentini… - Journal of peptide …, 2016 - Wiley Online Library
Liraglutide is a new generation lipopeptide drug used for the treatment of type II diabetes. In
this work, we describe new approaches for its preparation fully by chemical methods. The …

Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions

R Hussain, SE Harding, CS Hughes… - Biochemical Society …, 2016 - portlandpress.com
This article reviews current methods for the reliable heterologous overexpression in
Escherichia coli and purification of milligram quantities of bacterial membrane sensor kinase …