The appearance of genetically modified organisms on the food market a few years ago, and
the demand for more precise and reliable techniques to detect foreign (transgenic or …

Detection of harmful viruses and bacteria in plant material, vectors or natural reservoirs is
essential to ensure safe and sustainable agriculture. The techniques available have evolved …

Xylella fastidiosa is a regulated plant pathogen in many parts of the world. To increase
diagnostic capability of X. fastidiosa in the field, a loop-mediated isothermal amplification …

The polymerase chain reaction (PCR) has become an integral tool for the molecular
biologist. It represents a workhorse in the molecular biology laboratory whilst also proving to …

Rapid-cycle real-time polymerase chain reaction (PCR) methods may revolutionize the
manner in which plant pathogens are identified and diseases are diagnosed. As the …

A CRISPR/Cas12a based portable biosensor (Cas12a-PB) was developed to
simultaneously visually detect CaMV35S promoter and Lectin gene from genetically …

Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth
to real-time PCR. Real-time PCR-detection and expression analysis of gene (s) in real-time …

Phytoplasmas are cell wall-less bacteria inhabiting the phloem and utilizing it for their
spread. Infected plants often show changes in growth pattern and a reduced crop yield. A …

Real-time PCR technologies open increasing opportunities to detect and study
phytopathogenic and antagonistic fungi. They combine the sensitivity of conventional PCR …

Late wilt, a disease severely affecting maize fields throughout Israel, is characterized by the
relatively rapid wilting of maize plants from the tasseling stage to maturity. The disease is …