Lyophilization and development of solid protein pharmaceuticals

W Wang - International journal of pharmaceutics, 2000 - Elsevier
Developing recombinant protein pharmaceuticals has proved to be very challenging
because of both the complexity of protein production and purification, and the limited …

An overview of liposome lyophilization and its future potential

C Chen, D Han, C Cai, X Tang - Journal of controlled release, 2010 - Elsevier
Lyophilization is a promising approach to ensure the long-term stability of liposomes. After
decades of studies in this field, different lyoprotective mechanisms, such as water …

Developments in mid-infrared FT-IR spectroscopy of selected carbohydrates

M Kačuráková, RH Wilson - Carbohydrate polymers, 2001 - Elsevier
FT-IR spectroscopy has become a powerful research tool for elucidating the structure,
physical properties and interactions of carbohydrates. It provides a new interpretive and …

Trehalose as a “chemical chaperone” fact and fantasy

JH Crowe - Molecular aspects of the stress response: chaperones …, 2007 - Springer
Trehalose is a disaccharide of glucose that is found at high concentrations in a wide variety
of organisms that naturally survive drying in nature. Many years ago we reported that this …

Glass formation in plant anhydrobiotes: survival in the dry state

J Buitink, O Leprince - Cryobiology, 2004 - Elsevier
Anhydrobiotes can resist complete dehydration and survive the dry state for extended
periods of time. During drying, cytoplasmic viscosity increases dramatically and in the dry …

Lessons from nature: the role of sugars in anhydrobiosis

LM Crowe - Comparative Biochemistry and Physiology Part A …, 2002 - Elsevier
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A Fourier-transform infrared spectroscopy study of sugar glasses

WF Wolkers, AE Oliver, F Tablin, JH Crowe - Carbohydrate research, 2004 - Elsevier
Fourier-transform infrared spectroscopy (FTIR) was used to study the hydrogen-bonding
interactions that take place in vitrified carbohydrates of different chain lengths. The band …

Isolation and characterization of a D-7 LEA protein from pollen that stabilizes glasses in vitro

WF Wolkers, S McCready, WF Brandt… - … et Biophysica Acta (BBA …, 2001 - Elsevier
A heat-soluble protein present in substantial quantities in Typha latifolia pollen was purified
to homogeneity. The protein was subjected to cyanogen bromide cleavage, and the …

Vitrification is essential for anhydrobiosis in an African chironomid, Polypedilum vanderplanki

M Sakurai, T Furuki, K Akao… - Proceedings of the …, 2008 - National Acad Sciences
Anhydrobiosis is an extremely dehydrated state in which organisms show no detectable
metabolism but retain the ability to revive after rehydration. Thus far, two hypotheses have …

Drying without dying.

P Alpert, MJ Oliver - 2002 - cabidigitallibrary.org
This introductory chapter summarizes some of the current answers to questions (how do
plants survive desiccation? and how does surviving desiccation affect plant survival?) and …