The major facilitator superfamily (MFS) is the largest known superfamily of secondary active
transporters. MFS transporters are responsible for transporting a broad spectrum of …

To study integral membrane proteins, one has to extract them from the membrane—the step
that is typically achieved by the application of detergents. In this mini-review, we summarize …

Mass spectrometry (MS) of intact soluble protein complexes has emerged as a powerful
technique to study the stoichiometry, structure-function and dynamics of protein assemblies …

The altered activity of the fructose transporter GLUT5, an isoform of the facilitated-diffusion
glucose transporter family, has been linked to disorders such as type 2 diabetes and obesity …

Membrane proteins (MPs) play essential roles in numerous cellular processes. Because
around 70% of the currently marketed drugs target MPs, a detailed understanding of their …

Abstract Sodium/proton (Na+/H+) antiporters, located at the plasma membrane in every cell,
are vital for cell homeostasis. In humans, their dysfunction has been linked to diseases, such …

High cholesterol levels greatly increase the risk of cardiovascular disease. About 50 per cent
of cholesterol is eliminated from the body by its conversion into bile acids. However, bile …

Biological membranes define the boundaries of cells and compartmentalize the chemical
and physical processes required for life. Many biological processes are carried out by …

Protein stability in detergent or membrane-like environments is the bottleneck for structural
studies on integral membrane proteins (IMP). Irrespective of the method to study the …

Despite the growing importance of the mass spectrometry of membrane proteins, it is not
known how their transfer from solution into vacuum affects their stability and structure. To …