Understanding the invisible hands of sample preparation for cryo-EM
Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …
Disorders of lysosomal acidification—The emerging role of v-ATPase in aging and neurodegenerative disease
DJ Colacurcio, RA Nixon - Ageing research reviews, 2016 - Elsevier
Autophagy and endocytosis deliver unneeded cellular materials to lysosomes for
degradation. Beyond processing cellular waste, lysosomes release metabolites and ions …
degradation. Beyond processing cellular waste, lysosomes release metabolites and ions …
Determinants, maintenance, and function of organellar pH
SA Freeman, S Grinstein… - Physiological reviews, 2023 - journals.physiology.org
The protonation state of soluble and membrane-associated macromolecules dictates their
charge, conformation, and functional activity. In addition, protons (H+ or their equivalents) …
charge, conformation, and functional activity. In addition, protons (H+ or their equivalents) …
[HTML][HTML] An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology
Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …
Structures of a complete human V-ATPase reveal mechanisms of its assembly
Vesicular-or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton
pumps comprised of a cytoplasmic V 1 complex for ATP hydrolysis and a membrane …
pumps comprised of a cytoplasmic V 1 complex for ATP hydrolysis and a membrane …
Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase
J Zhao, S Benlekbir, JL Rubinstein - Nature, 2015 - nature.com
Abstract Eukaryotic vacuolar H+-ATPases (V-ATPases) are rotary enzymes that use energy
from hydrolysis of ATP to ADP to pump protons across membranes and control the pH of …
from hydrolysis of ATP to ADP to pump protons across membranes and control the pH of …
[PDF][PDF] Approaches to altering particle distributions in cryo-electron microscopy sample preparation
Cryo-electron microscopy (cryo-EM) can now be used to determine high-resolution structural
information on a diverse range of biological specimens. Recent advances have been driven …
information on a diverse range of biological specimens. Recent advances have been driven …
Breaking up and making up: The secret life of the vacuolar H+‐ATPase
RA Oot, S Couoh‐Cardel, S Sharma, NJ Stam… - Protein …, 2017 - Wiley Online Library
Abstract The vacuolar ATPase (V‐ATPase; V1Vo‐ATPase) is a large multisubunit proton
pump found in the endomembrane system of all eukaryotic cells where it acidifies the lumen …
pump found in the endomembrane system of all eukaryotic cells where it acidifies the lumen …
Molecular basis for the binding and modulation of V-ATPase by a bacterial effector protein
Intracellular pathogenic bacteria evade the immune response by replicating within host
cells. Legionella pneumophila, the causative agent of Legionnaires' Disease, makes use of …
cells. Legionella pneumophila, the causative agent of Legionnaires' Disease, makes use of …
The changing landscape of membrane protein structural biology through developments in electron microscopy
S Rawson, S Davies, JD Lippiat… - Molecular membrane …, 2016 - Taylor & Francis
Membrane proteins are ubiquitous in biology and are key targets for therapeutic
development. Despite this, our structural understanding has lagged behind that of their …
development. Despite this, our structural understanding has lagged behind that of their …