Structure and functional determinants of Rad6–Bre1 subunits in the histone H2B ubiquitin-conjugating complex

PK Shukla, JE Bissell, S Kumar, S Pokhrel… - Nucleic Acids …, 2023 - academic.oup.com
The conserved complex of the Rad6 E2 ubiquitin-conjugating enzyme and the Bre1 E3
ubiquitin ligase catalyzes histone H2B monoubiquitination (H2Bub1), which regulates …

H2B oncohistones cause homologous recombination defect and genomic instability through reducing H2B monoubiquitination in Schizosaccharomyces pombe

B Qin, G Lu, X Chen, C Zheng, H Lin, Q Liu… - Journal of Biological …, 2024 - ASBMB
Canonical oncohistones are histone H3 mutations in the N-terminal tail associated with
tumors and affect gene expression by altering H3 post-translational modifications (PTMs) …

Rapid purification of rabbit immunoglobulins using a single-step, negative-selection chromatography

PK Shukla, KS Radmall… - Protein Expression and …, 2023 - Elsevier
Custom polyclonal antibodies raised in rabbits are routinely used in immunoblotting and
other protein analysis techniques. Custom rabbit polyclonal antisera are generally purified …

Structure–function analysis of histone H2B and PCNA ubiquitination dynamics using deubiquitinase-deficient strains

KS Radmall, PK Shukla, AM Leng… - Scientific Reports, 2023 - nature.com
Post-translational covalent conjugation of ubiquitin onto proteins or ubiquitination is
important in nearly all cellular processes. Steady-state ubiquitination of individual proteins in …

[PDF][PDF] A system for in vivo evaluation of protein ubiquitination dynamics using deubiquitinase-deficient strains.

KS Radmall, PK Shukla, MB Chandrasekharan - researchgate.net
Post-translational covalent conjugation of ubiquitin onto proteins or ubiquitination is
important in nearly all cellular processes. Steady-state ubiquitination of individual proteins in …