Single-molecule kinetics in living cells

J Elf, I Barkefors - Annual review of biochemistry, 2019 - annualreviews.org
In the past decades, advances in microscopy have made it possible to study the dynamics of
individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be …

Single-molecule imaging and tracking of molecular dynamics in living cells

N Li, R Zhao, Y Sun, Z Ye, K He… - National Science …, 2017 - academic.oup.com
Unlike the ensemble-averaging measurements, the single-molecule imaging and tracking
(SMIT) in living cells provides the real-time quantitative information about the locations …

Nicotine induces morphological and functional changes in astrocytes via nicotinic receptor activity

SP Aryal, X Fu, JN Sandin, KR Neupane, JE Lakes… - Glia, 2021 - Wiley Online Library
Nicotine is a highly addictive compound present in tobacco, which causes the release of
dopamine in different regions of the brain. Recent studies have shown that astrocytes …

Selective fluorescent labeling of cellular proteins and its biological applications

JH Choi, S Kim, OY Kang, SY Choi, JY Hyun… - Chemical Society …, 2024 - pubs.rsc.org
Proteins, which are ubiquitous in cells and critical to almost all cellular functions, are
indispensable for life. Fluorescence imaging of proteins is key to understanding their …

Inverse Regulation of C–C Chemokine Receptor 3 Oligomerization by Downstream Proteins Indicates Biased Signal Transduction Pathways

J Li, Y Li, Y Ding, Y Song, J Li, H Chen… - The Journal of …, 2024 - ACS Publications
Oligomerization is one of the important mechanisms for G protein-coupled receptors
(GPCRs) to modulate their activity in signal transduction. However, details of how and why …

Cellular and Biophysical Applications of Genetic Code Expansion

HB Yi, S Lee, K Seo, H Kim, M Kim, HS Lee - Chemical Reviews, 2024 - ACS Publications
Despite their diverse functions, proteins are inherently constructed from a limited set of
building blocks. These compositional constraints pose significant challenges to protein …

Single-molecule imaging of protein interactions and dynamics

F Luo, G Qin, T Xia, X Fang - Annual Review of Analytical …, 2020 - annualreviews.org
Live-cell single-molecule fluorescence imaging has become a powerful analytical tool to
investigate cellular processes that are not accessible to conventional biochemical …

Imaging of receptor dimers in zebrafish and living cells via aptamer recognition and proximity-induced hybridization chain reaction

L Wang, W Li, J Sun, SY Zhang, S Yang, J Li… - Analytical …, 2018 - ACS Publications
On cell-membrane surfaces, receptor-protein dimers play fundamental roles in many
signaling pathways that are crucial for normal biological processes and cancer …

Oligomerization-Enhanced Receptor–Ligand Binding Revealed by Dual-Color Simultaneous Tracking on Living Cell Membranes

J Li, Y Ding, H Liu, H He, D Yu, X Wang… - The Journal of …, 2021 - ACS Publications
GPCR oligomerization plays a critical role in cellular signaling, yet the stoichiometry of the
interactions between oligomers and binding ligands in living cells remains a longstanding …

Brain region specific single-molecule fluorescence imaging

X Fu, FH Moonschi, AM Fox-Loe, AA Snell… - Analytical …, 2019 - ACS Publications
We developed an approach utilizing nanoscale vesicles extracted from brain regions
combined with single molecule imaging to monitor how an animal's physiological condition …