Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process
Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …
Factors involved in heterologous expression of proteins in E. coli host
M Pouresmaeil, S Azizi-Dargahlou - Archives of Microbiology, 2023 - Springer
The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …
biotechnology in the last century. These proteins are produced in the eukaryotic or …
Bacterial inclusion bodies are industrially exploitable amyloids
A De Marco, N Ferrer-Miralles… - FEMS microbiology …, 2019 - academic.oup.com
Understanding the structure, functionalities and biology of functional amyloids is an issue of
emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria …
emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria …
Catalytically-active inclusion bodies—Carrier-free protein immobilizates for application in biotechnology and biomedicine
Bacterial inclusion bodies (IBs) consist of unfolded protein aggregates and represent
inactive waste products often accumulating during heterologous overexpression of …
inactive waste products often accumulating during heterologous overexpression of …
Spatial and temporal distribution of ribosomes in single cells reveals aging differences between old and new daughters of Escherichia coli
Lineages of rod-shaped bacteria such as Escherichia coli exhibit a temporal decline in
elongation rate in a manner comparable to cellular or biological aging. The effect results …
elongation rate in a manner comparable to cellular or biological aging. The effect results …
TDP-43 inclusion bodies formed in bacteria are structurally amorphous, non-amyloid and inherently toxic to neuroblastoma cells
C Capitini, S Conti, M Perni, F Guidi, R Cascella… - PloS one, 2014 - journals.plos.org
Accumulation of ubiquitin-positive, tau-and α-synuclein-negative intracellular inclusions of
TDP-43 in the central nervous system represents the major hallmark correlated to …
TDP-43 in the central nervous system represents the major hallmark correlated to …
Biological role of bacterial inclusion bodies: a model for amyloid aggregation
E García‐Fruitós, R Sabate, NS De Groot… - The FEBS …, 2011 - Wiley Online Library
Inclusion bodies are insoluble protein aggregates usually found in recombinant bacteria
when they are forced to produce heterologous protein species. These particles are formed …
when they are forced to produce heterologous protein species. These particles are formed …
Influence of pH control in the formation of inclusion bodies during production of recombinant sphingomyelinase-D in Escherichia coli
A Castellanos-Mendoza, RM Castro-Acosta… - Microbial cell …, 2014 - Springer
Abstract Background Inclusion bodies (IBs) are aggregated proteins that form clusters when
protein is overexpressed in heterologous expression systems. IBs have been considered as …
protein is overexpressed in heterologous expression systems. IBs have been considered as …
Teaching an old pET new tricks: tuning of inclusion body formation and properties by a mixed feed system in E. coli
DJ Wurm, J Quehenberger, J Mildner… - Applied microbiology …, 2018 - Springer
Against the outdated belief that inclusion bodies (IBs) in Escherichia coli are only inactive
aggregates of misfolded protein, and thus should be avoided during recombinant protein …
aggregates of misfolded protein, and thus should be avoided during recombinant protein …
Bioprocessing of recombinant proteins from Escherichia coli inclusion bodies: insights from structure-function relationship for novel applications
The formation of inclusion bodies (IBs) during expression of recombinant therapeutic
proteins using E. coli is a significant hurdle in producing high-quality, safe, and efficacious …
proteins using E. coli is a significant hurdle in producing high-quality, safe, and efficacious …