Precision control of CRISPR-Cas9 using small molecules and light
The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas system is an
adaptive immune system of bacteria that has furnished several RNA-guided DNA …
adaptive immune system of bacteria that has furnished several RNA-guided DNA …
Enhanced safety and efficacy of protease-regulated CAR-T cell receptors
Regulatable CAR platforms could circumvent toxicities associated with CAR-T therapy, but
existing systems have shortcomings including leakiness and attenuated activity. Here, we …
existing systems have shortcomings including leakiness and attenuated activity. Here, we …
Synthetic transcription factor engineering for cell and gene therapy
B Bhatt, P García-Díaz, GW Foight - Trends in Biotechnology, 2024 - cell.com
Synthetic transcription factors (synTFs) that control beneficial transgene expression are an
important method to increase the safety and efficacy of cell and gene therapy. Reliance on …
important method to increase the safety and efficacy of cell and gene therapy. Reliance on …
Multidimensional control of therapeutic human cell function with synthetic gene circuits
Synthetic gene circuits that precisely control human cell function could expand the
capabilities of gene-and cell-based therapies. However, platforms for developing circuits in …
capabilities of gene-and cell-based therapies. However, platforms for developing circuits in …
High-performance multiplex drug-gated CAR circuits
Chimeric antigen receptor (CAR) T cells can revolutionize cancer medicine. However,
overactivation, lack of tumor-specific surface markers, and antigen escape have hampered …
overactivation, lack of tumor-specific surface markers, and antigen escape have hampered …
Multi-input chemical control of protein dimerization for programming graded cellular responses
Chemical and optogenetic methods for post-translationally controlling protein function have
enabled modulation and engineering of cellular functions. However, most of these methods …
enabled modulation and engineering of cellular functions. However, most of these methods …
Synthetic macromolecular switches for precision control of therapeutic cell functions
AP Teixeira, M Fussenegger - Nature Reviews Bioengineering, 2024 - nature.com
Cells rely on complex molecular networks to perceive and process external and internal
signals into tailored responses. These cellular abilities can be augmented and modified by …
signals into tailored responses. These cellular abilities can be augmented and modified by …
Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect
YF Chuang, AJ Phipps, FL Lin, V Hecht… - Cellular and Molecular …, 2021 - Springer
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-
associated (Cas) system provides a groundbreaking genetic technology that allows …
associated (Cas) system provides a groundbreaking genetic technology that allows …
Augmenting adaptive immunity: progress and challenges in the quantitative engineering and analysis of adaptive immune receptor repertoires
The adaptive immune system is a natural diagnostic sensor and therapeutic. It recognizes
threats earlier than clinical symptoms manifest and neutralizes antigens with exquisite …
threats earlier than clinical symptoms manifest and neutralizes antigens with exquisite …
Design and engineering of genetically encoded protein biosensors for small molecules
AC Leonard, TA Whitehead - Current Opinion in Biotechnology, 2022 - Elsevier
Highlights•Ligand-responsive biosensors are integral to cellular-control applications.•
Integrated biosensors connect ligand recognition to a single output signal.•Portable …
Integrated biosensors connect ligand recognition to a single output signal.•Portable …