Single C-to-T substitution using engineered APOBEC3G-nCas9 base editors with minimum genome-and transcriptome-wide off-target effects
Cytosine base editors (CBEs) enable efficient cytidine-to-thymidine (C-to-T) substitutions at
targeted loci without double-stranded breaks. However, current CBEs edit all Cs within their …
targeted loci without double-stranded breaks. However, current CBEs edit all Cs within their …
Engineered deaminases as a key component of DNA and RNA editing tools
L Budzko, K Hoffa-Sobiech, P Jackowiak… - … Therapy-Nucleic Acids, 2023 - cell.com
Over recent years, zinc-dependent deaminases have attracted increasing interest as key
components of nucleic acid editing tools that can generate point mutations at specific sites in …
components of nucleic acid editing tools that can generate point mutations at specific sites in …
Structural insights into APOBEC3-mediated lentiviral restriction
KA Delviks-Frankenberry, BA Desimmie, VK Pathak - Viruses, 2020 - mdpi.com
Mammals have developed clever adaptive and innate immune defense mechanisms to
protect against invading bacterial and viral pathogens. Human innate immunity is …
protect against invading bacterial and viral pathogens. Human innate immunity is …
Dysregulated APOBEC3G causes DNA damage and promotes genomic instability in multiple myeloma
Multiple myeloma (MM) is a heterogeneous disease characterized by significant genomic
instability. Recently, a causal role for the AID/APOBEC deaminases in inducing somatic …
instability. Recently, a causal role for the AID/APOBEC deaminases in inducing somatic …
Crystal structure of the catalytic domain of HIV-1 restriction factor APOBEC3G in complex with ssDNA
The human APOBEC3G protein is a cytidine deaminase that generates cytidine to deoxy-
uridine mutations in single-stranded DNA (ssDNA), and capable of restricting replication of …
uridine mutations in single-stranded DNA (ssDNA), and capable of restricting replication of …
Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions
Abstract Background Cytidine base editors (CBEs), composed of a cytidine deaminase
fused to Cas9 nickase (nCas9), enable efficient C-to-T conversion in various organisms …
fused to Cas9 nickase (nCas9), enable efficient C-to-T conversion in various organisms …
APOBEC alteration contributes to tumor growth and immune escape in pan-cancer
H Guo, L Zhu, L Huang, Z Sun, H Zhang, B Nong… - Cancers, 2022 - mdpi.com
Simple Summary The APOBEC3 family (apolipoprotein B mRNA editing enzyme catalytic
polypeptide-like) was shown to induce tumor mutations through an aberrant DNA editing …
polypeptide-like) was shown to induce tumor mutations through an aberrant DNA editing …
Single-stranded DNA damage: Protecting the single-stranded DNA from chemical attack
R Anindya - DNA repair, 2020 - Elsevier
Cellular processes, such as DNA replication, recombination and transcription, require DNA
strands separation and single-stranded DNA is formation. The single-stranded DNA is …
strands separation and single-stranded DNA is formation. The single-stranded DNA is …
Insights into the structures and multimeric status of APOBEC proteins involved in viral restriction and other cellular functions
XS Chen - Viruses, 2021 - mdpi.com
Apo lipoprotein B mRNA e diting c atalytic polypeptide-like (APOBEC) proteins belong to a
family of deaminase proteins that can catalyze the deamination of cytosine to uracil on …
family of deaminase proteins that can catalyze the deamination of cytosine to uracil on …
Inhibition of base editors with anti-deaminases derived from viruses
Cytosine base editors (CBEs), combining cytidine deaminases with the Cas9 nickase
(nCas9), enable targeted C-to-T conversions in genomic DNA and are powerful genome …
(nCas9), enable targeted C-to-T conversions in genomic DNA and are powerful genome …