Sortase A is a membrane enzyme responsible for the anchoring of surface-exposed proteins
to the cell wall envelope of Gram-positive bacteria. As a well-studied member of the sortase …

Gram-positive bacteria use sortase cysteine transpeptidase enzymes to covalently attach
proteins to their cell wall and to assemble pili. In pathogenic bacteria sortases are potential …

We report systematic quantum mechanics‐only (QM‐only) and QM/molecular mechanics
(MM) calculations on an enzyme‐catalyzed reaction to assess the convergence behavior of …

Bacteria possess complex and varying cell walls with many surface exposed proteins.
Sortases are responsible for the covalent attachment of specific proteins to the …

The cell wall is a critical extracellular barrier for bacteria and many other organisms. In
bacteria, this structural layer consists of peptidoglycan, which maintains cell shape and …

Experimental evidence for enzymatic mechanisms is often scarce, and in many cases
inadvertently biased by the employed methods. Thus, apparently contradictory model …

Bioorthogonal, chemoselective ligation methods are an essential part of the tools utilized to
investigate biochemical pathways. Specifically enzymatic approaches are valuable methods …

With continuous emergence and widespread of multidrug-resistant Staphylococcus aureus
infections, common antibiotics have become ineffective in treating these infections in the …

ABSTRACT A fundamental step of Gram-positive pathogenesis is the bacterial adhesion to
the host tissue involving interaction between bacterial surface molecules and host ligands …

Staphylococcus aureus is the leading cause of hospital‐acquired infections in the U nited S
tates. The emergence of multidrug‐resistant strains of S. aureus has created an urgent need …