Past, present, and future of affinity-based cell separation technologies

K Bacon, A Lavoie, BM Rao, M Daniele, S Menegatti - Acta biomaterialia, 2020 - Elsevier
Progress in cell purification technology is critical to increase the availability of viable cells for
therapeutic, diagnostic, and research applications. A variety of techniques are now available …

Phase diagrams of aqueous two-phase systems formed by polyethylene glycol+ ammonium sulfate+ water: equilibrium data and thermodynamic modeling

GF Murari, JA Penido, PAL Machado, LR de Lemos… - Fluid Phase …, 2015 - Elsevier
Phase diagrams of aqueous two-phase systems (ATPS) composed of polyethylene glycol
(PEG) of different molar mass (400 or 10,000 or 35,000) g mol− 1+ ammonium sulfate+ …

Measurement and correlation of phase equilibria in aqueous two-phase systems containing polyethyleneglycol (2 000, 4 000, and 6 000) and sulfate salts …

FHB Sosa, D de Araujo Sampaio, FO Farias… - Fluid Phase …, 2017 - Elsevier
Liquid–liquid equilibrium (LLE) data were obtained for polyethylene glycol (PEG)-salt
aqueous two-phase system (ATPS) at 298.15, 318.15 and 338.15 K. Two salts, copper …

Generation of polyethylene glycol-dextran aqueous two-phase system droplets using different culture media under in vitro conditions

K Chairez-Cantu, M González-González… - Food and Bioproducts …, 2023 - Elsevier
Aqueous two-phase systems (ATPS) involve the combination of two immiscible polymers
that at certain concentrations form a biphasic separation system. Due to the physicochemical …

Characterization of polymer‐polymer aqueous two‐phase system droplets for 3D culture future applications

K Chairez‐Cantu… - Journal of Chemical …, 2023 - Wiley Online Library
BACKGROUND Cell‐based high‐throughput platforms using aqueous two‐phase systems
(ATPS) have led to the construction of 3D cultures using ATPS in which stem cells are …

Influence of tie line length and volume ratio on the partition behavior of peripheral blood and conjugated CD34 antibody in polymer-polymer aqueous two-phase …

M Nisslein, M González-González… - Separation and …, 2021 - Elsevier
Aqueous two-phase systems (ATPS) offer great opportunities regarding the purification of
stem cells for future medical applications. However, most publications exploring the use of …

Continuous aqueous two-phase extraction: From microfluidics to integrated biomanufacturing

D Ferreira-Faria, MR Aires-Barros, AM Azevedo - Fluid Phase Equilibria, 2020 - Elsevier
Aqueous two-phase extraction (ATPE) has been used since the 1960s, but even having
advantages as high recovery yields and selectivity and relatively low cost, it has not been …

Elimination of contaminants from cell preparations using aqueous two-phase partitioning

M González-González, RC Willson… - Separation and …, 2016 - Elsevier
Contamination by cell debris and non-viable cells is common in cell-based processes. Cell
fragments and dead cells can be produced during culture and in purification steps, and often …

Cell‐based aqueous two‐phase systems for therapeutics

M González‐González… - Journal of Chemical …, 2020 - Wiley Online Library
Cell‐based aqueous two‐phase system technologies present an emerging and promising
translational tool for the development of next‐generation therapeutic applications …

Aqueous two‐phase systems in Latin America: perspective and future trends

M González‐González, G Picó, ÁS Lima… - Journal of Chemical …, 2022 - Wiley Online Library
Aqueous two‐phase systems (ATPS) have been demonstrated to be a simple and robust
worldwide applied bioprocessing technology. From high value microscopic to macroscopic …