Crotoxin B: heterologous expression, protein folding, immunogenic properties, and irregular presence in crotalid venoms

MA Mejía-Sánchez, H Clement, LL Corrales-García… - Toxins, 2022 - mdpi.com
Crotoxin complex CA/CB and crotamine are the main toxins associated with Crotalus
envenomation besides the enzymatic activities of phospholipases (PLA2) and proteases …

cDNA Cloning, Heterologous Expression, Cytotoxicity, and Inhibitory Effects of a Disintegrin from Bothrops ammodytoides Venom

H Clement, LL Corrales-García… - International Journal of …, 2023 - Springer
An mRNA transcript that codes for a Disintegrin and a Metalloprotease from a venom gland
of the viper Bothrops ammodytoides was isolated. Vector pCR® 2.1-TOPO was used to …

[HTML][HTML] Synergism of in vitro plasmodicidal activity of phospholipase A2 isoforms isolated from panamanian Bothrops asper venom

R Simões-Silva, JJ Alfonso, AF Gómez… - Chemico-Biological …, 2021 - Elsevier
Bothrops asper is one of the most important snake species in Central America, mainly
because of its medical importance in countries like Ecuador, Panama and Costa Rica …

Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A2 from Malaysian Spitting Cobra (Naja sumatrana) Venom

NAH Abdullah, MRA Rusmili, SA Zainal Abidin… - Toxins, 2021 - mdpi.com
Phospholipase A2 (PLA2) toxins are one of the main toxin families found in snake venom.
PLA2 toxins are associated with various detrimental effects, including neurotoxicity …

Structures and functions of crotoxin-like heterodimers and acidic phospholipases A2 from Gloydius intermedius venom: insights into the origin of neurotoxic-type …

ZM Yang, Q Guo, ZR Ma, Y Chen, ZZ Wang… - Journal of …, 2015 - Elsevier
The cDNAs encoding four major phospholipases A 2 (PLA 2 s) were sequenced while the
expressed sequence tags of Gloydius intermedius venom glands were constructed. These …

[HTML][HTML] Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from …

LM Resende, JR Almeida, R Schezaro-Ramos… - Toxicon, 2017 - Elsevier
Abstract Phospholipases A 2 (PLA 2 s) constitute a class of extensively studied toxins,
isolated from snake venoms. Basic PLA 2 isoforms mediate various toxicological effects …

cDNA cloning, heterologous expression, protein folding and immunogenic properties of a phospholipase A2 from Bothrops ammodytoides venom

H Clement, G Corzo, E Neri-Castro, I Arenas… - Protein Expression and …, 2019 - Elsevier
A mRNA transcript that codes for a phospholipase (PLA 2) was isolated from a single venom
gland of the Bothrops ammodytoides viper. The PLA 2 transcript was cloned onto a pCR® …

Antiplatelet and anticoagulant activities of two phospholipase A2s purified from Cerastes cerastes venom: Structure‐function relationship

C Fatah, S Samah, LD Fatima - Journal of biochemical and …, 2018 - Wiley Online Library
This study aimed to elucidate anticoagulant/antiplatelet mechanisms of two previously
purified PLA2s from Cerastes cerastes venom, here, termed Cc1‐PLA2 and Cc2‐PLA2. Both …

Immunogenic Properties of Recombinant Enzymes from Bothrops ammodytoides towards the Generation of Neutralizing Antibodies against Its Own Venom

H Clement, LL Corrales-García, D Bolaños, G Corzo… - Toxins, 2019 - mdpi.com
Bothropic venoms contain enzymes such as metalloproteases, serine-proteases, and
phospholipases, which acting by themselves, or in synergism, are the cause of the …

Neutralization of “Chaco eagle”(Buteogallus coronatus) serum on some activities of Bothrops spp. venoms

PI Regner, MD Saggese, VC de Oliveira, LC Lanari… - Toxicon, 2022 - Elsevier
Several species of reptiles and mammals have components in their sera that can neutralize
toxic components present in snake venoms. In this manuscript, we studied the neutralizing …