A basic guide to real time PCR in microbial diagnostics: definitions, parameters, and everything

P Kralik, M Ricchi - Frontiers in microbiology, 2017 - frontiersin.org
Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection,
quantification, and typing of different microbial agents in the areas of clinical and veterinary …

Microbial quality and safety of milk and milk products in the 21st century

V Fusco, D Chieffi, F Fanelli… - … Reviews in Food …, 2020 - Wiley Online Library
Milk and milk products have been utilized by humans for many thousands of years. With the
advent of metagenomic studies, our knowledge on the microbiota of milk and milk products …

Molecular approaches to analysing the microbial composition of raw milk and raw milk cheese

L Quigley, O O'Sullivan, TP Beresford, RP Ross… - International journal of …, 2011 - Elsevier
The availability and application of culture-independent tools that enable a detailed
investigation of the microbiota and microbial biodiversity of food systems has had a major …

Detection of viable Mycobacterium avium subspecies paratuberculosis in powdered infant formula by phage-PCR and confirmed by culture

G Botsaris, BMC Swift, I Slana, M Liapi… - International journal of …, 2016 - Elsevier
Surveys from different parts of the world have reported that viable Mycobacterium avium
subsp. paratuberculosis (MAP) can be cultured from approximately 2% of samples of retail …

Mycobacterium avium subsp. paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatment

P Kralik, A Nocker, I Pavlik - International journal of food microbiology, 2010 - Elsevier
Mycobacterium avium subsp. paratuberculosis (MAP) is known to be a very slow-growing
organism. The fact that cells typically need several weeks to form visible colonies severely …

Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk

ZE Gerrard, BMC Swift, G Botsaris, RS Davidson… - Food …, 2018 - Elsevier
A survey of retail purchased semi-skimmed pasteurised milk (n= 368) for Mycobacterium
avium subspecies paratuberculosis (MAP) was conducted between May 2014 and June …

[HTML][HTML] Mycobacterium avium subsp. paratuberculosis in dairy products, meat, and drinking water

CO Gill, L Saucier, WJ Meadus - Journal of Food Protection, 2011 - Elsevier
Mycobacterium avium subsp. paratuberculosis (Map) is the cause of Johne's disease, a
chronic infection of the gut, in ruminant animals that provide milk and/or meat for human …

Mycobacterium avium subspecies paratuberculosis – an important food borne pathogen of high public health significance with special reference to India: an update

KK Chaubey, SV Singh, S Gupta, M Singh… - Veterinary …, 2017 - Taylor & Francis
This review underlines the public health significance of 'Indian Bison Type'of Mycobacterium
avium subspecies paratuberculosis (MAP) and also its potential as 'zoonotic infection'. In the …

Lining the small intestine with mycobacteriophages protects from Mycobacterium avium subsp. paratuberculosis and eliminates fecal shedding

VK Harman-McKenna, R Eshraghisamani… - Proceedings of the …, 2024 - pnas.org
Johne's disease (JD), a chronic, infectious enteritis caused by Mycobacterium avium subsp.
paratuberculosis (MAP), affects wild and domestic ruminants. There is no cure or effective …

Sheep as a potential source of bovine TB: epidemiology, pathology and evaluation of diagnostic techniques

M Muñoz‐Mendoza, B Romero… - Transboundary and …, 2016 - Wiley Online Library
Bovine tuberculosis (TB) infection is infrequently diagnosed in sheep. Most reports are from
single individual cases or flock outbreaks. However, in Spain several outbreaks have been …