DNA transposition at work
AB Hickman, F Dyda - Chemical reviews, 2016 - ACS Publications
DNA transposons are defined segments of DNA that are able to move from one genomic
location to another. Movement is facilitated by one or more proteins, called the transposase …
location to another. Movement is facilitated by one or more proteins, called the transposase …
Precise cut-and-paste DNA insertion using engineered type VK CRISPR-associated transposases
CJ Tou, B Orr, BP Kleinstiver - Nature Biotechnology, 2023 - nature.com
CRISPR-associated transposases (CASTs) enable recombination-independent, multi-
kilobase DNA insertions at RNA-programmed genomic locations. However, the utility of type …
kilobase DNA insertions at RNA-programmed genomic locations. However, the utility of type …
Transposable phage mu
RM Harshey - Mobile DNA III, 2015 - Wiley Online Library
Transposable phage Mu has played a historic role in the development of the mobile DNA
element field (1). The very first paper that christened this phage after its mu tator properties …
element field (1). The very first paper that christened this phage after its mu tator properties …
Target site selection and remodelling by type V CRISPR-transposon systems
Canonical CRISPR–Cas systems provide adaptive immunity against mobile genetic
elements. However, type IF, IB and VK systems have been adopted by Tn7-like transposons …
elements. However, type IF, IB and VK systems have been adopted by Tn7-like transposons …
Selective TnsC recruitment enhances the fidelity of RNA-guided transposition
Bacterial transposons are pervasive mobile genetic elements that use distinct DNA-binding
proteins for horizontal transmission. For example, Escherichia coli Tn 7 homes to a specific …
proteins for horizontal transmission. For example, Escherichia coli Tn 7 homes to a specific …
Mechanistic details of CRISPR-associated transposon recruitment and integration revealed by cryo-EM
JU Park, AWL Tsai, TH Chen… - Proceedings of the …, 2022 - National Acad Sciences
CRISPR-associated transposons (CASTs) are Tn7-like elements that are capable of RNA-
guided DNA integration. Although structural data are known for nearly all core transposition …
guided DNA integration. Although structural data are known for nearly all core transposition …
Mechanism of target site selection by type VK CRISPR-associated transposases
CRISPR-associated transposases (CASTs) repurpose nuclease-deficient CRISPR effectors
to catalyze RNA-guided transposition of large genetic payloads. Type VK CASTs offer …
to catalyze RNA-guided transposition of large genetic payloads. Type VK CASTs offer …
Structural basis for DNA targeting by the Tn7 transposon
Tn7 transposable elements are unique for their highly specific, and sometimes
programmable, target-site selection mechanisms and precise insertions. All the elements in …
programmable, target-site selection mechanisms and precise insertions. All the elements in …
Structural basis for target site selection in RNA-guided DNA transposition systems
CRISPR-associated transposition systems allow guide RNA–directed integration of a single
DNA cargo in one orientation at a fixed distance from a programmable target sequence. We …
DNA cargo in one orientation at a fixed distance from a programmable target sequence. We …
Mechanistic insights into transposon cleavage and integration by TnsB of ShCAST system
T Zeng, J Yin, Z Liu, Z Li, Y Zhang, Y Lv, ML Lu, M Luo… - Cell Reports, 2023 - cell.com
The type VK CRISPR-associated transposons (CASTs) allow RNA-guided DNA integration
and have great potential as a programmable site-specific gene insertion tool. Although all …
and have great potential as a programmable site-specific gene insertion tool. Although all …