In the past 15 years, there have been rapid developments in the ability to cotranslationally
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …

Interrogating biological systems is often limited by access to biological probes. The
emergence of “click chemistry” has revolutionized bioconjugate chemistry by providing facile …

The study of biomolecules in their native environments is a challenging task because of the
vast complexity of cellular systems. Technologies developed in the last few years for the …

Dissecting complex cellular processes requires the ability to track biomolecules as they
function within their native habitat. Although genetically encoded tags such as GFP are …

Polymers that can respond to external stimuli are of great interest in medicine, especially as
controlled drug release vehicles. In this critical review, we consider the types of stimulus …

Expansion of the genetic code with nonstandard amino acids (nsAAs) has enabled
biosynthesis of proteins with diverse new chemistries. However, this technology has been …

We describe a general and rapid route for the addition of unnatural amino acids to the
genetic code of Saccharomyces cerevisiae. Five amino acids have been incorporated into …

In recent years, a number of bioorthogonal reactions have been developed, exemplified by
click chemistry, that enable the efficient formation of a specific product, even within a highly …

Although chemists can synthesize virtually any small organic molecule, our ability to
rationally manipulate the structures of proteins is quite limited, despite their involvement in …

The matrix metalloproteinases (MMP)-2 and-9, also known as the gelatinases have been
long recognized as major contributors to the proteolytic degradation of extracellular matrix …