Integrative deep learning framework predicts lipidomics-based investigation of preservatives on meat nutritional biomarkers and metabolic pathways

W Jia, A Guo, W Bian, R Zhang, X Wang… - Critical Reviews in Food …, 2023 - Taylor & Francis
Preservatives are added as antimicrobial agents to extend the shelf life of meat. Adding
preservatives to meat products can affect their flavor and nutrition. This review clarifies the …

Effect of concentrated salts solutions on the stability of immobilized enzymes: Influence of inactivation conditions and immobilization protocol

SA Braham, EH Siar, S Arana-Peña, D Carballares… - Molecules, 2021 - mdpi.com
This paper aims to investigate the effects of some salts (NaCl,(NH4) 2SO4 and Na2SO4) at
pH 5.0, 7.0 and 9.0 on the stability of 13 different immobilized enzymes: five lipases, three …

Promotion effect of salt on intramuscular neutral lipid hydrolysis during dry-salting process of porcine (biceps femoris) muscles by inducing phosphorylation of ATGL …

J Pan, S Zhao, L He, M Zhang, C Li, S Huang, J Wang… - Food Chemistry, 2022 - Elsevier
Towards a better understanding of the formation mechanism of salt on intramuscular
triglyceride (TG) hydrolysis occurring in biceps femoris (BF) muscles during dry-salting …

Structural Characterization of Functionally Important Chloride Binding Sites in the Marine Vibrio Alkaline Phosphatase

S Markússon, JG Hjorleifsson, P Kursula… - Biochemistry, 2022 - ACS Publications
Enzyme stability and function can be affected by various environmental factors, such as
temperature, pH, and ionic strength. Enzymes that are located outside the relatively …

Enhanced permeability of recombinant E. coli cells with deep eutectic solvent for transformation of rutin

F Zhang, CT Zhu, QM Peng, FQ Wang… - Journal of Chemical …, 2020 - Wiley Online Library
BACKGROUND Whole‐cell catalysis has been widely used because the steps of extracting
pure enzyme are omitted and the cost would be greatly saved. However, the efficiency of the …

Towards a mechanistic model of oxidase deactivation in a bubble column

AV Høst, JM Woodley, AS Bommarius - Chemical Engineering Science, 2024 - Elsevier
Despite the many advantages of biocatalysis, enzyme stability remains an issue. This work
investigates the long-term kinetic stability of a water-forming NAD (P) H-oxidase (NOX2), an …

A chloride ring is an ancient evolutionary innovation mediating the assembly of the collagen IV scaffold of basement membranes

V Pedchenko, R Bauer, EN Pokidysheva… - Journal of Biological …, 2019 - ASBMB
Collagen IV scaffold is a principal component of the basement membrane (BM), a
specialized extracellular matrix that is essential for animal multicellularity and tissue …

[HTML][HTML] X-ray crystal structure of Vibrio alkaline phosphatase with the non-competitive inhibitor cyclohexylamine

B Ásgeirsson, S Markússon, SS Hlynsdóttir… - … and Biophysics Reports, 2020 - Elsevier
Background Para-nitrophenyl phosphate, the common substrate for alkaline phosphatase
(AP), is available as a cyclohexylamine salt. Here, we report that cyclohexylamine is a non …

Chloride promotes refolding of active Vibrio alkaline phosphatase through an inactive dimeric intermediate with an altered interface

JG Hjörleifsson, B Ásgeirsson - FEBS Open bio, 2019 - Wiley Online Library
Most enzymes are homodimers or higher order multimers. Cold‐active alkaline phosphatase
from Vibrio splendidus (VAP) transitions into a dimer with very low activity under mild …

The high catalytic rate of the cold‐active Vibrio alkaline phosphatase requires a hydrogen bonding network involving a large interface loop

JG Hjörleifsson, R Helland, M Magnúsdóttir… - FEBS Open …, 2021 - Wiley Online Library
The role of surface loops in mediating communication through residue networks is still a
relatively poorly understood part in the study of cold adaptation of enzymes, especially in …