Cryo-electron tomography is the only technique that can provide sub-nanometer resolved
images of cell regions or even whole cells, without the need of labeling or staining methods …

Electron tomography (ET) has emerged as a powerful technique to address fundamental
questions in molecular and cellular biology. It makes possible visualization of the molecular …

Cutting‐edge biophysical technologies including total internal reflection fluorescence
microscopy, single molecule fluorescence, single channel opening events, fluorescence …

The necessity of detection and identification of damages in structural elements intensifies
the development of non-destructive testing (NDT) methods and techniques suitable for …

In recent years, electron tomography has improved our three-dimensional (3D) insight in the
structural architecture of cells and organelles. For studies that involve the 3D imaging of …

Tomograms of biological specimens derived using transmission electron microscopy can be
intrinsically noisy due to the use of low electron doses, the presence of a “missing wedge” in …

Ratio imaging is playing an increasingly important role in modern cell biology. Combined
with ratiometric dyes or fluorescence resonance energy transfer (FRET) biosensors, the …

Trabecular bone structure and bone density contribute to the strength of bone and are
important in the study of osteoporosis. Wavelets are a powerful tool in characterizing and …

Ceramide-1-phosphate (C1P) is a novel bioactive sphingolipid formed by the
phosphorylation of ceramide catalyzed by ceramide kinase (CERK). In this study, we …

The crucial problem inherent to electron tomography is radiation damage or, related to this,
the choice of the correct electron dose: an excessive dose destroys the specimen, especially …