The genetically encoded fluorescent sensors convert chemical and physical signals into
light. They are powerful tools for the visualisation of physiological processes in living cells …

Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences
are often underestimated. Damage to cellular macromolecules upon excitation light …

The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …

We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record
brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet …

We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with
fast and complete maturation, as well as record brightness, quantum yield (75%) and …

Over the past 20 years, protein engineering has been extensively used to improve and
modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting …

Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …

The insertion of precise genetic modifications by genome editing tools such as CRISPR-
Cas9 is limited by the relatively low efficiency of homology-directed repair (HDR) compared …

The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has
revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of …

Uncovering the mechanisms of virus infection and assembly is crucial for preventing the
spread of viruses and treating viral disease. The technique of single-virus tracking (SVT) …