Responding to chemical gradients: bacterial chemotaxis

V Sourjik, NS Wingreen - Current opinion in cell biology, 2012 - Elsevier
Chemotaxis allows bacteria to follow gradients of nutrients and other environmental stimuli.
The bacterium Escherichia coli performs chemotaxis via a run-and-tumble strategy in which …

The acetate switch

AJ Wolfe - Microbiology and molecular biology reviews, 2005 - Am Soc Microbiol
To succeed, many cells must alternate between life-styles that permit rapid growth in the
presence of abundant nutrients and ones that enhance survival in the absence of those …

General calibration of microbial growth in microplate readers

K Stevenson, AF McVey, IBN Clark, PS Swain… - Scientific reports, 2016 - nature.com
Optical density (OD) measurements of microbial growth are one of the most common
techniques used in microbiology, with applications ranging from studies of antibiotic efficacy …

The anti-Shine–Dalgarno sequence drives translational pausing and codon choice in bacteria

GW Li, E Oh, JS Weissman - Nature, 2012 - nature.com
Protein synthesis by ribosomes takes place on a linear substrate but at non-uniform speeds.
Transient pausing of ribosomes can affect a variety of co-translational processes, including …

Escherichia coli Physiology in Luria-Bertani Broth

G Sezonov, D Joseleau-Petit, R d'Ari - Journal of bacteriology, 2007 - Am Soc Microbiol
ABSTRACT Luria-Bertani broth supports Escherichia coli growth to an optical density at 600
nm (OD600) of 7. Surprisingly, however, steady-state growth ceases at an OD600 of 0.3 …

Metabolic control of persister formation in Escherichia coli

SM Amato, MA Orman, MP Brynildsen - Molecular cell, 2013 - cell.com
Bacterial persisters are phenotypic variants that form from the action of stress response
pathways triggering toxin-mediated antibiotic tolerance. Although persisters form during …

Chemotaxis as a navigation strategy to boost range expansion

J Cremer, T Honda, Y Tang, J Wong-Ng, M Vergassola… - Nature, 2019 - nature.com
Bacterial chemotaxis, the directed movement of cells along gradients of chemoattractants, is
among the best-characterized subjects in molecular biology,,,,,,,,–, but much less is known …

The Complete Genome Sequence of Escherichia coli DH10B: Insights into the Biology of a Laboratory Workhorse

T Durfee, R Nelson, S Baldwin, G Plunkett III… - Journal of …, 2008 - Am Soc Microbiol
Escherichia coli DH10B was designed for the propagation of large insert DNA library clones.
It is used extensively, taking advantage of properties such as high DNA transformation …

Nitrogen assimilation in Escherichia coli: putting molecular data into a systems perspective

WC van Heeswijk, HV Westerhoff… - … and Molecular Biology …, 2013 - Am Soc Microbiol
We present a comprehensive overview of the hierarchical network of intracellular processes
revolving around central nitrogen metabolism in Escherichia coli. The hierarchy intertwines …

Conversion of proteins into biofuels by engineering nitrogen flux

YX Huo, KM Cho, JGL Rivera, E Monte, CR Shen… - Nature …, 2011 - nature.com
Biofuels are currently produced from carbohydrates and lipids in feedstock. Proteins, in
contrast, have not been used to synthesize fuels because of the difficulties of deaminating …