Protein crystallography using synchrotron radiation sources has had a tremendous impact
on biology, having yielded the structures of thousands of proteins and given detailed insight …

Highlights•Common data quality indicators grouped as primary, secondary, or
wrong/misleading.•A gedanken experiment reveals shortcomings of some common …

Following integration of the observed diffraction spots, the process ofdata reduction'initially
aims to determine the point-group symmetry of the data and the likely space group. This can …

Folate receptors (FRα, FRβ and FRγ) are cysteine-rich cell-surface glycoproteins that bind
folate with high affinity to mediate cellular uptake of folate. Although expressed at very low …

Flaviviruses, the human pathogens responsible for dengue fever, West Nile fever, tick-borne
encephalitis, and yellow fever, are endemic in tropical and temperate parts of the world. The …

Determining new protein structures from X-ray diffraction data at low resolution or with a
weak anomalous signal is a difficult and often an impossible task. Here we propose a …

Secondary active transporters exploit the electrochemical potential of solutes to shuttle
specific substrate molecules across biological membranes, usually against their …

The number of membrane protein structures in the Protein Data Bank is becoming significant
and growing. Here, the transmembrane domain structures of the helical membrane proteins …

Abstract The 70-kilodalton (kDa) heat-shock proteins (Hsp70s) are ubiquitous molecular
chaperones essential for cellular protein folding and proteostasis. Each Hsp70 has two …

Membrane proteins perform a host of vital cellular functions. Deciphering the molecular
mechanisms whereby they fulfill these functions requires detailed biophysical and structural …