Dynamic nuclear polarization for sensitivity enhancement in biomolecular solid-state NMR

T Biedenbänder, V Aladin, S Saeidpour… - Chemical …, 2022 - ACS Publications
Solid-state NMR with magic-angle spinning (MAS) is an important method in structural
biology. While NMR can provide invaluable information about local geometry on an atomic …

[HTML][HTML] Dynamic nuclear polarization for sensitivity enhancement in modern solid-state NMR

ASL Thankamony, JJ Wittmann, M Kaushik… - Progress in nuclear …, 2017 - Elsevier
The field of dynamic nuclear polarization has undergone tremendous developments and
diversification since its inception more than 6 decades ago. In this review we provide an in …

Biomolecular and biological applications of solid-state NMR with dynamic nuclear polarization enhancement

WY Chow, G De Paëpe, S Hediger - Chemical Reviews, 2022 - ACS Publications
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

High-resolution NMR studies of antibiotics in cellular membranes

J Medeiros-Silva, S Jekhmane, AL Paioni… - Nature …, 2018 - nature.com
The alarming rise of antimicrobial resistance requires antibiotics with unexploited
mechanisms. Ideal templates could be antibiotics that target the peptidoglycan precursor …

[HTML][HTML] INEPT and CP transfer efficiencies of dynamic systems in MAS solid-state NMR

K Aebischer, M Ernst - Journal of Magnetic Resonance, 2024 - Elsevier
Hartmann–Hahn cross polarization and INEPT polarization transfer are the most popular
sequences to increase the polarization of low-γ nuclei in magic-angle spinning solid-state …

High-field dynamic nuclear polarization

B Corzilius - Annual review of physical chemistry, 2020 - annualreviews.org
Dynamic nuclear polarization (DNP) is one of the most prominent methods of sensitivity
enhancement in nuclear magnetic resonance (NMR). Even though solid-state DNP under …

New applications of solid-state NMR in structural biology

PCA van der Wel - Emerging topics in life sciences, 2018 - portlandpress.com
Various recent developments in solid-state nuclear magnetic resonance (ssNMR)
spectroscopy have enabled an array of new insights regarding the structure, dynamics, and …

Determining Cholesterol Binding to Membrane Proteins by Cholesterol 13C Labeling in Yeast and Dynamic Nuclear Polarization NMR

MR Elkins, IV Sergeyev, M Hong - Journal of the American …, 2018 - ACS Publications
We present a general strategy for determining the cholesterol-binding site of eukaryotic
membrane proteins in native-like lipid membranes by NMR spectroscopy. The strategy …

Primary transfer step in the light-driven ion pump bacteriorhodopsin: An irreversible U-turn revealed by dynamic nuclear polarization-enhanced magic angle spinning …

QZ Ni, TV Can, E Daviso, M Belenky… - Journal of the …, 2018 - ACS Publications
Despite much attention, the path of the highly consequential primary proton transfer in the
light-driven ion pump bacteriorhodopsin (bR) remains mysterious. Here we use DNP …

Insights into protein misfolding and aggregation enabled by solid-state NMR spectroscopy

PCA van der Wel - Solid state nuclear magnetic resonance, 2017 - Elsevier
The aggregation of proteins and peptides into a variety of insoluble, and often non-native,
aggregated states plays a central role in many devastating diseases. Analogous processes …