Advances in promoter engineering: novel applications and predefined transcriptional control

AP Cazier, J Blazeck - Biotechnology Journal, 2021 - Wiley Online Library
Synthetic biology continues to progress by relying on more robust tools for transcriptional
control, of which promoters are the most fundamental component. Numerous studies have …

De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts

Y Xu, X Wang, C Zhang, X Zhou, X Xu, L Han… - Nature …, 2022 - nature.com
High-sugar diet causes health problems, many of which can be addressed with the use of
sugar substitutes. Rubusoside and rebaudiosides are interesting molecules, considered the …

GET pathway mediates transfer of mislocalized tail-anchored proteins from mitochondria to the ER

S Matsumoto, S Ono, S Shinoda, C Kakuta… - Journal of Cell …, 2022 - rupress.org
Tail-anchored (TA) membrane proteins have a potential risk to be mistargeted to the
mitochondrial outer membrane (OM). Such mislocalized TA proteins can be extracted by the …

A layered genetic design enables the yeast galactose regulon to respond to cyanamide

J Song, J Fan, C Fan, N He, X Ye, M Cao… - ACS Synthetic …, 2023 - ACS Publications
The commonly used expression systems in Saccharomyces cerevisiae typically rely on
either constitutive or galactose-regulated promoters. The lack of inducible systems in S …

Photothermally modulated magnetic nanochains as swarm nanorobotics for microreaction control

Y Liao, D Liu, B Wang, Z Wu, M Ni… - ACS Applied Nano …, 2022 - ACS Publications
The intensification of microreactions is important for biological and chemical reactions with
microliter volumes as reaction units, including microliter bioassay or protein synthesis. At the …

Alleviation of the Byproducts Formation Enables Highly Efficient Biosynthesis of Rosmarinic Acid in Saccharomyces cerevisiae

P Zhou, C Yue, Y Zhang, Y Li, X Da… - Journal of Agricultural …, 2022 - ACS Publications
Rosmarinic acid as a polyphenolic compound has great values in the pharmaceutical,
cosmetic, and food industries. To achieve efficient biosynthesis of rosmarinic acid, the major …

[HTML][HTML] Cyanamide-inducible expression of homing nuclease I− SceI for selectable marker removal and promoter characterisation in Saccharomyces cerevisiae

L McDonnell, S Evans, Z Lu, M Suchoronczak… - Synthetic and Systems …, 2024 - Elsevier
In synthetic biology, microbial chassis including yeast Saccharomyces cerevisiae are
iteratively engineered with increasing complexity and scale. Wet-lab genetic engineering …

Cyanamide-inducible expression of homing nuclease I-SceI for iterative genome engineering and parallel promoter characterisation in Saccharomyces cerevisiae

L McDonnell, S Evans, Z Lu, M Suchoronczak… - bioRxiv, 2024 - biorxiv.org
In synthetic biology, microbial chasses including yeast Saccharomyces cerevisiae are
iteratively engineered with increasing complexity and scale. Wet-lab genetic engineering …

Assigning roles in Chlamydomonas ribosome biogenesis: The conserved factor NIP7

RF Gutierrez, H Ciol, ALC Barra, DA Leonardo… - … et Biophysica Acta (BBA …, 2024 - Elsevier
Ribosome biogenesis (RB) is a highly conserved process across eukaryotes that results in
the assembly of functional ribosomal subunits. Studies in Saccharomyces cerevisiae and …

Uniform [13C,15N]-labeled and glycosylated IgG1 Fc expressed in Saccharomyces cerevisiae

AR Davis, ET Roberts, IJ Amster, AW Barb - Journal of biomolecular NMR, 2024 - Springer
Despite the prevalence and importance of glycoproteins in human biology, methods for
isotope labeling suffer significant limitations. Common prokaryotic platforms do not produce …