Cell-free gene expression: methods and applications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …
molecules and processes outside of living intact cells. The foundational principle is that …
Constructing cell-free expression systems for low-cost access
Cell-free systems for gene expression have gained attention as platforms for the facile study
of genetic circuits and as highly effective tools for teaching. Despite recent progress, the …
of genetic circuits and as highly effective tools for teaching. Despite recent progress, the …
Synthetic biology education and pedagogy: a review of evolving practices in a growing discipline
Synthetic biology is a growing field with an increasing number of successful applications.
Yet, synthetic biology (SynBio) education initiatives are underreported and disconnected …
Yet, synthetic biology (SynBio) education initiatives are underreported and disconnected …
At-home, cell-free synthetic biology education modules for transcriptional regulation and environmental water quality monitoring
As the field of synthetic biology expands, the need to grow and train science, technology,
engineering, and math (STEM) practitioners is essential. However, the lack of access to …
engineering, and math (STEM) practitioners is essential. However, the lack of access to …
Cell-Free Translation Quantification via a Fluorescent Minihelix
Cell-free gene expression systems are used in numerous applications, including medicine
making, diagnostics, and educational kits. Accurate quantification of nonfluorescent proteins …
making, diagnostics, and educational kits. Accurate quantification of nonfluorescent proteins …
Characterizing and Improving Reaction Times for E. coli-Based Cell-Free Protein Synthesis
LR Burrington, KR Watts, JP Oza - ACS Synthetic Biology, 2021 - ACS Publications
Cell-free protein synthesis (CFPS) is a platform biotechnology that has enabled the on-
demand synthesis of proteins for a variety of applications. Numerous advances have …
demand synthesis of proteins for a variety of applications. Numerous advances have …
Activation of energy metabolism through growth media reformulation enables a 24-hour workflow for cell-free expression
Cell-free protein synthesis (CFPS) platforms have undergone numerous workflow
improvements to enable diverse applications in research, biomanufacturing, and education …
improvements to enable diverse applications in research, biomanufacturing, and education …
Characterizing and improving pET vectors for cell-free expression
K Jew, PEJ Smith, B So, J Kasman, JP Oza… - … in Bioengineering and …, 2022 - frontiersin.org
Cell-free protein synthesis (CFPS) is an in vitro process that enables diverse applications in
research, biomanufacturing, point-of-care diagnostics, therapeutics, and education using …
research, biomanufacturing, point-of-care diagnostics, therapeutics, and education using …
Analysis of the innovation trend in cell-free synthetic biology
Cell-free synthetic biology is a maturing field that aims to assemble biomolecular reactions
outside cells for compelling applications in drug discovery, metabolic engineering …
outside cells for compelling applications in drug discovery, metabolic engineering …
Development of Solid-State Storage for Cell-Free Expression Systems
AW Brookwell, JL Gonzalez, AW Martinez… - ACS Synthetic …, 2023 - ACS Publications
The fragility of biological systems during storage, transport, and utilization necessitates
reliable cold-chain infrastructure and limits the potential of biotechnological applications. In …
reliable cold-chain infrastructure and limits the potential of biotechnological applications. In …