Cell-free gene expression: an expanded repertoire of applications
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …
It has been used for more than 50 years across the life sciences as a foundational research …
Microfluidics for medical diagnostics and biosensors
This article reviews the recent development in microfluidics for medical diagnostics and
integrations with biosensors. Diagnostic and sensing applications have been the focus of …
integrations with biosensors. Diagnostic and sensing applications have been the focus of …
Latest developments in micro total analysis systems
A Arora, G Simone, GB Salieb-Beugelaar… - Analytical …, 2010 - ACS Publications
The field of micro total analysis systems (μTAS)(1) or lab on a chip (LOC) has extended its
usefulness into many new fields and disciplines spanning basic research to commercial …
usefulness into many new fields and disciplines spanning basic research to commercial …
Bottom-up construction of complex biomolecular systems with cell-free synthetic biology
N Laohakunakorn, L Grasemann… - … in bioengineering and …, 2020 - frontiersin.org
Cell-free systems offer a promising approach to engineer biology since their open nature
allows for well-controlled and characterized reaction conditions. In this review, we discuss …
allows for well-controlled and characterized reaction conditions. In this review, we discuss …
Miniaturization through lab-on-a-chip: Utopia or reality for routine laboratories? A review
Á Ríos, M Zougagh, M Avila - Analytica chimica acta, 2012 - Elsevier
Micro total analysis (μTAS), also called “lab-on-a-chip (LOC)” technology, promises
solutions for high throughput and highly specific analysis for chemistry, biology and …
solutions for high throughput and highly specific analysis for chemistry, biology and …
Implementation of cell-free biological networks at steady state
H Niederholtmeyer, V Stepanova… - Proceedings of the …, 2013 - National Acad Sciences
Living cells maintain a steady state of biochemical reaction rates by exchanging energy and
matter with the environment. These exchanges usually do not occur in in vitro systems …
matter with the environment. These exchanges usually do not occur in in vitro systems …
Cell-free supplement mixtures: Elucidating the history and biochemical utility of additives used to support in vitro protein synthesis in E. coli extract
Cell-free protein synthesis (CFPS) has become an established biotechnology tool for rapid
protein expression. Over the past few decades many advances have elevated CFPS from a …
protein expression. Over the past few decades many advances have elevated CFPS from a …
Droplet-driven transports on superhydrophobic-patterned surface microfluidics
Droplet-based transport phenomena driven by surface tension have been explored as an
automated pumping source for a number of chemical and biological applications. In this …
automated pumping source for a number of chemical and biological applications. In this …
Experiment and mathematical modeling of gene expression dynamics in a cell-free system
T Stögbauer, L Windhager, R Zimmer… - Integrative …, 2012 - academic.oup.com
Cell-free in vitro expression is increasingly important for high-throughput expression
screening, high yield protein production and synthetic biology applications. Yet its potential …
screening, high yield protein production and synthetic biology applications. Yet its potential …
Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks
The construction of a minimal cell that exhibits the essential characteristics of life is a great
challenge in the field of synthetic biology. Assembling a minimal cell requires …
challenge in the field of synthetic biology. Assembling a minimal cell requires …