Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples

G Lear, I Dickie, J Banks, S Boyer, HL Buckley… - New Zealand Journal of …, 2018 - JSTOR
Advances in the sequencing of DNA extracted from media such as soil and water offer huge
opportunities for biodiversity monitoring and assessment, particularly where the collection or …

Development of chitosan-coated nanoemulsions of two sulfides present in onion (Allium cepa) essential oil and their nematicidal activities against the pine wood …

E Yang, JW Lee, PS Chang, IK Park - Environmental Science and …, 2021 - Springer
Pine wood nematode, Bursaphelenchus xylophilus, is a plant parasitic nematode which
causes severe damage to several Pinus species. Two natural compounds, dipropyl trisulfide …

Developing a Real-Time PCR Assay for Direct Identification and Quantification of Pratylenchus penetrans in Soil

R Baidoo, G Yan, S Nagachandrabose… - Plant …, 2017 - Am Phytopath Society
The root-lesion nematode Pratylenchus penetrans is a major pathogen of potato worldwide.
Yield losses may be exacerbated by interaction with the fungus Verticillium dahliae in the …

Methods for nematode extraction.

N Viaene, J Hallmann… - Techniques for work with …, 2021 - cabidigitallibrary.org
Nematodes can be present in different matrices. This chapter describes several methods to
extract nematodes from soil and plant parts. It is crucial that an appropriate method is …

The added value of proficiency tests: choosing the proper method for extracting Meloidogyne second-stage juveniles from soil

L den Nijs, W van den Berg - Nematology, 2013 - brill.com
To gain insight into the different extraction processes used in laboratories in various
European countries and the effect these methods might have on the number of Meloidogyne …

Developing a Real-Time PCR Assay for Direct Identification and Quantification of Soybean Cyst Nematode, Heterodera glycines, in Soil and Its Discrimination from Sugar Beet …

R Baidoo, G Yan - Plant Disease, 2021 - Am Phytopath Society
The soybean cyst nematode (SCN) Heterodera glycines continues to be a major threat to
soybean production worldwide. Morphological discrimination between SCN and other …

Use of Chemical Flocculation and Nested PCR for Heterodera glycines Detection in DNA Extracts from Field Soils with Low Population Densities

R Baidoo, G Yan, B Nelson, AM Skantar… - Plant disease, 2017 - Am Phytopath Society
The soybean cyst nematode (SCN) Heterodera glycines is a major pathogen of soybean
worldwide. Distinction between SCN and other members of the H. schachtii sensu stricto …

Biodiversity and efficacy of fungal isolates associated with Kenyan populations of potato cyst nematode (Globodera spp.)

MN Nyang'au, KS Akutse, K Fathiya, MK Charimbu… - Biological Control, 2023 - Elsevier
Abstract Potato Cyst Nematodes (PCN)(Globodera rostochiensis and G. pallida Woll.) are
quarantine pests of potato (Solanum tuberosum L.) worldwide capable of causing significant …

[HTML][HTML] Quantitative relationship of soil texture with the observed population density reduction of Heterodera glycines after annual corn rotation in Nebraska

O Pérez-Hernández, LJ Giesler - Journal of Nematology, 2014 - ncbi.nlm.nih.gov
Soil texture has been commonly associated with the population density of Heterodera
glycines (soybean cyst nematode: SCN), but such an association has been mainly …

Assessment of Factors Associated with Molecular Quantification of Stubby Root Nematode Paratrichodorus allius from Field Soil DNA

D Huang, G Yan, NC Gudmestad, J Whitworth - Plant disease, 2019 - Am Phytopath Society
Factors relating to SYBR Green-based quantitative real-time PCR (qPCR) quantification of
stubby root nematode Paratrichodorus allius using soil DNA were evaluated in this study …