Integrating immunology and microfluidics for single immune cell analysis

N Sinha, N Subedi, J Tel - Frontiers in immunology, 2018 - frontiersin.org
The field of immunoengineering aims to develop novel therapies and modern vaccines to
manipulate and modulate the immune system and applies innovative technologies toward …

Follicle-stimulating hormone receptor: advances and remaining challenges

F De Pascali, A Tréfier, F Landomiel, V Bozon… - International review of …, 2018 - Elsevier
Follicle-stimulating hormone (FSH) is produced in the pituitary and is essential for
reproduction. It specifically binds to a membrane receptor (FSHR) expressed in somatic cells …

Microfluidic chip for precise trapping of single cells and temporal analysis of signaling dynamics

N Sinha, H Yang, D Janse, L Hendriks… - Communications …, 2022 - nature.com
Microfluidic designs are versatile examples of technology miniaturisation that find their
applications in various cell biology research, especially to investigate the influence of …

Rapid lipolytic oscillations in ex vivo adipose tissue explants revealed through microfluidic droplet sampling at high temporal resolution

J Hu, X Li, RL Judd, CJ Easley - Lab on a Chip, 2020 - pubs.rsc.org
Our understanding of adipose tissue biology has steadily evolved. While structural and
energy storage functionalities have been in the forefront, a key endocrine role for adipocytes …

Dynamic generation of concentration-and temporal-dependent chemical signals in an integrated microfluidic device for single-cell analysis

AM Gonzalez-Suarez, JG Peña-del Castillo… - Analytical …, 2018 - ACS Publications
Intracellular signaling pathways are affected by the temporal nature of external chemical
signaling molecules such as neurotransmitters or hormones. Developing high-throughput …

Engineering global and local signal generators for probing temporal and spatial cellular signaling dynamics

H Yang, J Tel - Frontiers in Bioengineering and Biotechnology, 2023 - frontiersin.org
Cells constantly encounter a wide range of environmental signals and rely on their signaling
pathways to initiate reliable responses. Understanding the underlying signaling …

[HTML][HTML] PiFlow: A biocompatible low-cost programmable dynamic flow pumping system utilizing a Raspberry Pi Zero and commercial piezoelectric pumps

T Kassis, PM Perez, CJW Yang, LR Soenksen… - HardwareX, 2018 - Elsevier
With the rise of research utilizing microphysiological systems (MPSs), the need for tools that
enable the physiological mimicking of the relevant cellular environment is vital. The limited …

Dynamic microfluidic cytometry for single-cell cellomics: High-throughput probing single-cell-resolution signaling

P Chen, S Yan, J Wang, Y Guo, Y Dong… - Analytical …, 2018 - ACS Publications
Cell signaling is a fast, dynamic, and complex process, which controls a variety of critical
physiological functions. Methods to investigate such dynamic information, however, suffer …

A two-pulse cellular stimulation test elucidates variability and mechanisms in signaling pathways

M Sumit, A Jovic, RR Neubig, S Takayama… - Biophysical …, 2019 - cell.com
Mammalian cells respond in a variable manner when provided with physiological pulses of
ligand, such as low concentrations of acetylcholine present for just tens of seconds or TNFα …

The MAPK/ERK channel capacity exceeds 6 bit/hour

P Nałęcz-Jawecki, PA Gagliardi… - PLoS computational …, 2023 - journals.plos.org
Living cells utilize signaling pathways to sense, transduce, and process information. As the
extracellular stimulation often has rich temporal characteristics which may govern dynamic …