Gene essentiality is a founding concept of genetics with important implications in both
fundamental and applied research. Multiple screens have been performed over the years in …

Mitochondria contain∼ 1,000 different proteins, most of which are imported from the cytosol.
Two import pathways that direct proteins into the mitochondrial inner membrane and matrix …

INTRODUCTION Genetic interactions occur when mutations in two or more genes combine
to generate an unexpected phenotype. An extreme negative or synthetic lethal genetic …

Although the genes essential for life have been identified in less complex model organisms,
their elucidation in human cells has been hindered by technical barriers. We used extensive …

The nucleolus is the most prominent membraneless condensate in the nucleus. It comprises
hundreds of proteins with distinct roles in the rapid transcription of ribosomal RNA (rRNA) …

Large-scale genetic analysis of lethal phenotypes has elucidated the molecular
underpinnings of many biological processes. Using the bacterial clustered regularly …

To determine whether genes retain ancestral functions over a billion years of evolution and
to identify principles of deep evolutionary divergence, we replaced 414 essential yeast …

Nearly all mitochondrial proteins are encoded by the nuclear genome and imported into
mitochondria after synthesis on cytosolic ribosomes. These precursor proteins are …

Formation of single-strand DNA (ssDNA) tails at a double-strand break (DSB) is a key step in
homologous recombination and DNA-damage signaling. The enzyme (s) producing ssDNA …

Dysfunction of protein quality control contributes to the cellular pathology of polyglutamine
(polyQ) expansion diseases and other neurodegenerative disorders associated with …