Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Fluorescence microscopy, in particular immunofluorescence microscopy, has been used
extensively for the assessment of kidney function and pathology for both research and …

Fluorescence lifetime imaging microscopy (FLIM) is used in diverse disciplines, including
biology, chemistry and biophysics, but its use has been limited by the complexity of the data …

For nearly a century developmental biologists have recognized that cells from embryos can
differ in their potential to differentiate into distinct cell types. Recently, it has been recognized …

The non-invasive high-resolution spatial mapping of cell metabolism within tissues could
provide substantial advancements in assessing the efficacy of stem cell therapy and …

DNA damage signaling is critical for the maintenance of genome integrity and cell fate
decision. Poly (ADP-ribose) polymerase 1 (PARP1) is a DNA damage sensor rapidly …

There is a lack of fast and high resolution methods to measure metabolic activity of single
cells in their native environment. Here we develop a straightforward, non-invasive and …

In the stem cell field there is a lack of non invasive and fast methods to identify stem cell's
metabolic state, differentiation state and cell-lineage commitment. Here we describe a label …

Presence of reactive oxygen species (ROS) in excess of normal physiological level results in
oxidative stress. This can lead to a range of pathological conditions including inflammation …

Purpose: Human pluripotent stem cell (hPSC)-derived retinal organoids are a platform for
investigating retinal development, pathophysiology, and cellular therapies. In contrast to …