Eukaryotic chromatin is kept flexible and dynamic to respond to environmental, metabolic,
and developmental cues through the action of a family of so-called “nucleosome …

Regulation of gene expression in eukaryotes is an extremely complex process. In this
review, we break down several critical steps, emphasizing new data and techniques that …

RNA polymerase II (Pol II) must break the nucleosomal barrier to gain access to DNA and
transcribe genes efficiently. New single-molecule techniques have elucidated many …

Specification of Sox2+ proneurosensory progenitors within otic ectoderm is a prerequisite for
the production of sensory cells and neurons for hearing. However, the underlying molecular …

It has been some 40 years since repeating subunits in eukaryotic chromatin, initially termed
“nu bodies,” were described. Four decades of study have characterized the structural …

RNA polymerase II (PolII) transcribes RNA within a chromatin context, with nucleosomes
acting as barriers to transcription. Despite these barriers, transcription through chromatin in …

Nucleosomal DNA is thought to be generally inaccessible to DNA-binding factors, such as
micrococcal nuclease (MNase). Here, we digest Drosophila chromatin with high and low …

RSC and SWI/SNF are related ATP-dependent chromatin remodeling machines that move
nucleosomes, regulating access to DNA. We addressed their roles in nucleosome phasing …

Nucleosome repositioning at gene promoters is a fundamental aspect of the regulation of
gene expression. However, the extent to which nucleosome repositioning is used within …

Specific cell states in metazoans are established by the symphony of gene expression
programs that necessitate intricate synergic interactions between transcription factors and …