Macromolecules change their shape (conformation) in the process of carrying out their
functions. The imaging by cryo-electron microscopy of rapidly-frozen, individual copies of …

Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is
an interdisciplinary field that takes advantage of advances in biochemistry, physics, and …

Cryogenic electron tomography (cryo-ET) allows structural determination of biomolecules in
their native environment (in situ). Its potential of providing information on the dynamics of …

ContinuousFlex is a user-friendly open-source software package for analyzing continuous
conformational variability of macromolecules in cryo electron microscopy (cryo-EM) and cryo …

Resolving the structural variability of proteins is often key to understanding the structure–
function relationship of those macromolecular machines. Single particle analysis using …

Structural biology has evolved greatly due to the advances introduced in fields like electron
microscopy. This image-capturing technique, combined with improved algorithms and …

Single-particle analysis by electron microscopy is a well established technique for analyzing
the three-dimensional structures of biological macromolecules. Besides its ability to produce …

Xmipp is an open-source software package consisting of multiple programs for processing
data originating from electron microscopy and electron tomography, designed and managed …

There is a variety of experimental and computational techniques available to explore protein
dynamics, each presenting advantages and limitations. One promising experimental …

Abstract Hybrid Electron Microscopy Normal Mode Analysis (HEMNMA) method was
introduced in 2014. HEMNMA computes normal modes of a reference model (an atomic …