Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: a review

Z Yang, Z Zhang - Biotechnology advances, 2018 - Elsevier
Pichia pastoris has been recognized as one of the most industrially important hosts for
heterologous protein production. Despite its high protein productivity, the optimization of P …

Biotechnological production of carotenoids by yeasts: an overview

LC Mata-Gómez, JC Montañez, A Méndez-Zavala… - Microbial cell …, 2014 - Springer
Nowadays, carotenoids are valuable molecules in different industries such as chemical,
pharmaceutical, poultry, food and cosmetics. These pigments not only can act as vitamin A …

[HTML][HTML] Metabolic engineering of Pichia pastoris

DA Peña, B Gasser, J Zanghellini, MG Steiger… - Metabolic …, 2018 - Elsevier
Besides its use for efficient production of recombinant proteins the methylotrophic yeast
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …

Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and Applications

V Juturu, JC Wu - ChemBioChem, 2018 - Wiley Online Library
Pichia pastoris is a well‐known platform strain for heterologous protein expression. Over the
past five years, different strategies to improve the efficiency of recombinant protein …

Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae

MI Espinosa, RA Gonzalez-Garcia, K Valgepea… - Nature …, 2020 - nature.com
Utilising one-carbon substrates such as carbon dioxide, methane, and methanol is vital to
address the current climate crisis. Methylotrophic metabolism enables growth and energy …

[HTML][HTML] Anthocyanins, multi-functional natural products of industrial relevance: Recent biotechnological advances

T Belwal, G Singh, P Jeandet, A Pandey, L Giri… - Biotechnology …, 2020 - Elsevier
Anthocyanins, the color compounds of plants, are known for their wide applications in food,
nutraceuticals and cosmetic industry. The biosynthetic pathway of anthocyanins is well …

[HTML][HTML] Development of synthetic biology tools to engineer Pichia pastoris as a chassis for the production of natural products

J Gao, L Jiang, J Lian - Synthetic and systems biotechnology, 2021 - Elsevier
The methylotrophic yeast Pichia pastoris (aka Komagataella phaffii) is one of the most
commonly used hosts for industrial production of recombinant proteins. As a non …

Identifying and engineering the ideal microbial terpenoid production host

S Moser, H Pichler - Applied Microbiology and Biotechnology, 2019 - Springer
More than 70,000 different terpenoid structures are known so far; many of them offer highly
interesting applications as pharmaceuticals, flavors and fragrances, or biofuels. Extraction of …

Carotenoids and their biosynthesis in fungi

G Sandmann - Molecules, 2022 - mdpi.com
Carotenoids represent a class of pigmented terpenoids. They are distributed in all taxonomic
groups of fungi. Most of the fungal carotenoids differ in their chemical structures to those …

Towards systems metabolic engineering in Pichia pastoris

JP Schwarzhans, T Luttermann, M Geier… - Biotechnology …, 2017 - Elsevier
The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of
recombinant proteins, frequently outperforming other heterologous hosts. Already, a …