Protecting Proteins from Desiccation Stress Using Molecular Glasses and Gels

GI Olgenblum, BO Hutcheson, GJ Pielak… - Chemical …, 2024 - ACS Publications
Faced with desiccation stress, many organisms deploy strategies to maintain the integrity of
their cellular components. Amorphous glassy media composed of small molecular solutes or …

Trehalose: chemical structure, biological functions, and practical application

EP Feofilova, AI Usov, IS Mysyakina, GA Kochkina - Microbiology, 2014 - Springer
Up-to-date information concerning the chemical structure and properties of trehalose, its
natural occurrence and biological functions in plants, fungi, and prokaryotes, as well as its …

Unraveling protein stabilization mechanisms: Vitrification and water replacement in a glass transition temperature controlled system

N Grasmeijer, M Stankovic, H de Waard… - … et Biophysica Acta (BBA …, 2013 - Elsevier
The aim of this study was to elucidate the role of the two main mechanisms used to explain
the stabilization of proteins by sugar glasses during drying and subsequent storage: the …

Properties of aqueous trehalose mixtures: glass transition and hydrogen bonding

GI Olgenblum, L Sapir, D Harries - Journal of chemical theory and …, 2020 - ACS Publications
Trehalose is a naturally occurring disaccharide known to remarkably stabilize
biomacromolecules in the biologically active state. The stabilizing effect is typically observed …

Role of saccharides on thermal stability of phycocyanin in aqueous solutions

M Faieta, L Neri, G Sacchetti, A Di Michele… - Food Research …, 2020 - Elsevier
The effect of heat and equivalent thermal effect (CT ref z) on discolouration of Spirulina
algae extracts in water, sucrose and trehalose solutions at different concentration was …

Growth and nitrogen fixation in Lotus japonicus and Medicago truncatula under NaCl stress: nodule carbon metabolism

M López, JA Herrera-Cervera, C Iribarne… - Journal of Plant …, 2008 - Elsevier
Lotus japonicus and Medicago truncatula model legumes, which form determined and
indeterminate nodules, respectively, provide a convenient system to study plant–Rhizobium …

Internal dynamics and protein–matrix coupling in trehalose-coated proteins

L Cordone, G Cottone, S Giuffrida, G Palazzo… - … Et Biophysica Acta (BBA …, 2005 - Elsevier
We review recent studies on the role played by non-liquid, water-containing matrices on the
dynamics and structure of embedded proteins. Two proteins were studied, in water …

Intracellular viscosity: Methods of measurement and role in metabolism

EO Puchkov - … (Moscow) Supplement Series A: Membrane and Cell …, 2013 - Springer
This review is devoted to the study of intracellular viscosity. Methods of intracellular viscosity
measurement in cell populations and single cells are characterized and critically evaluated …

Boar spermatozoa cryopreservation in low glycerol/trehalose enriched freezing media improves cellular integrity

O Gutiérrez-Pérez, M de Lourdes Juárez-Mosqueda… - Cryobiology, 2009 - Elsevier
The use of glycerol for boar semen cryopreservation results in low fertility, possibly due to
toxicity. This has led to recommend the use of solutions with less than 4% glycerol …

Trehalose as a stabilizer of the lipid composition of membranes and the composition of the cytosol of frozen/thawed rooster spermatozoa

O Stanishevskaya, Y Silyukova, V Tereshina… - Agriculture, 2023 - mdpi.com
Low-temperature semen storage technologies are already being used in poultry
conservation programs, but the quality of reproductive material stored in cryobanks varies …