CRISPR-based diagnostics

MM Kaminski, OO Abudayyeh, JS Gootenberg… - Nature Biomedical …, 2021 - nature.com
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …

[HTML][HTML] Diagnostics for SARS-CoV-2 infections

BD Kevadiya, J Machhi, J Herskovitz, MD Oleynikov… - Nature materials, 2021 - nature.com
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread to nearly every
corner of the globe, causing societal instability. The resultant coronavirus disease 2019 …

Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants

NL Welch, M Zhu, C Hua, J Weller, ME Mirhashemi… - Nature medicine, 2022 - nature.com
Abstract The coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear
need for high-throughput, multiplexed and sensitive assays for detecting severe acute …

A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution

T Zhang, R Deng, Y Wang, C Wu, K Zhang… - Nature Biomedical …, 2022 - nature.com
The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has
highlighted the need for versatile diagnostic assays that can discriminate among emerging …

COVID-19: Discovery, diagnostics and drug development

T Asselah, D Durantel, E Pasmant, G Lau… - Journal of …, 2021 - Elsevier
Summary Coronavirus disease 2019 (COVID-19) started as an epidemic in Wuhan in 2019,
and has since become a pandemic. Groups from China identified and sequenced the virus …

Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants

J Arizti-Sanz, AD Bradley, YB Zhang… - Nature biomedical …, 2022 - nature.com
The widespread transmission and evolution of severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) call for rapid nucleic acid diagnostics that are easy to use …

Rapid electrochemical detection of coronavirus SARS-CoV-2

T Chaibun, J Puenpa, T Ngamdee… - Nature …, 2021 - nature.com
Abstract Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 …

Magnetic beads combined with carbon black-based screen-printed electrodes for COVID-19: A reliable and miniaturized electrochemical immunosensor for SARS …

L Fabiani, M Saroglia, G Galatà, R De Santis… - Biosensors and …, 2021 - Elsevier
The diffusion of novel SARS-CoV-2 coronavirus over the world generated COVID-19
pandemic event as reported by World Health Organization on March 2020. The huge issue …

Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification

Z Xu, D Chen, T Li, J Yan, J Zhu, T He, R Hu… - Nature …, 2022 - nature.com
Fast, inexpensive, and multiplexed detection of multiple nucleic acids is of great importance
to human health, yet it still represents a significant challenge. Herein, we propose a nucleic …

High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects

H Tong, J Huang, Q Xiao, B He, X Dong, Y Liu… - Nature …, 2023 - nature.com
CRISPR–Cas13 systems have recently been used for targeted RNA degradation in various
organisms. However, collateral degradation of bystander RNAs has limited their in vivo …