[HTML][HTML] Paramagnetic NMR in solution and the solid state

AJ Pell, G Pintacuda, CP Grey - Progress in nuclear magnetic resonance …, 2019 - Elsevier
The field of paramagnetic NMR has expanded considerably in recent years. This review
addresses both the theoretical description of paramagnetic NMR, and the way in which it is …

Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules …

GM Clore, J Iwahara - Chemical reviews, 2009 - ACS Publications
Understanding the function of biological macromolecules and their complexes at the
physicochemical level requires knowledge of both their structure and dynamics …

Cancer mutations of the tumor suppressor SPOP disrupt the formation of active, phase-separated compartments

JJ Bouchard, JH Otero, DC Scott, E Szulc, EW Martin… - Molecular cell, 2018 - cell.com
Mutations in the tumor suppressor SPOP (speckle-type POZ protein) cause prostate, breast,
and other solid tumors. SPOP is a substrate adaptor of the cullin3-RING ubiquitin ligase and …

Phospho-dependent phase separation of FMRP and CAPRIN1 recapitulates regulation of translation and deadenylation

TH Kim, B Tsang, RM Vernon, N Sonenberg, LE Kay… - Science, 2019 - science.org
Membraneless organelles involved in RNA processing are biomolecular condensates
assembled by phase separation. Despite the important role of intrinsically disordered protein …

Sequence determinants of the conformational properties of an intrinsically disordered protein prior to and upon multisite phosphorylation

EW Martin, AS Holehouse, CR Grace… - Journal of the …, 2016 - ACS Publications
Many cell signaling events are coordinated by intrinsically disordered protein regions (IDRs)
that undergo multisite Serine/Threonine phosphorylation. The conformational properties of …

Interaction hot spots for phase separation revealed by NMR studies of a CAPRIN1 condensed phase

TH Kim, BJ Payliss, ML Nosella… - Proceedings of the …, 2021 - National Acad Sciences
The role of biomolecular condensates in regulating biological function and the importance of
dynamic interactions involving intrinsically disordered protein regions (IDRs) in their …

13C Direct Detected NMR for Challenging Systems

IC Felli, R Pierattelli - Chemical Reviews, 2022 - ACS Publications
Thanks to recent improvements in NMR spectrometer hardware and pulse sequence design,
modern 13C NMR has become a useful tool for biomolecular applications. The complete …

The ambivalent role of proline residues in an intrinsically disordered protein: from disorder promoters to compaction facilitators

B Mateos, C Conrad-Billroth, M Schiavina… - Journal of molecular …, 2020 - Elsevier
Intrinsically disordered proteins (IDPs) carry out many biological functions. They lack a
stable three-dimensional structure, but rather adopt many different conformations in dynamic …

Altered ceramide metabolism is a feature in the extracellular vesicle-mediated spread of alpha-synuclein in Lewy body disorders

M Kurzawa-Akanbi, S Tammireddy, I Fabrik… - Acta …, 2021 - Springer
Mutations in glucocerebrosidase (GBA) are the most prevalent genetic risk factor for Lewy
body disorders (LBD)—collectively Parkinson's disease, Parkinson's disease dementia and …

NMR experiments for studies of dilute and condensed protein phases: application to the phase-separating protein CAPRIN1

LE Wong, TH Kim, DR Muhandiram… - Journal of the …, 2020 - ACS Publications
Intrinsically disordered proteins (IDPs) or regions of intrinsic disorder in otherwise folded
proteins (IDRs) play important roles in many different biological processes, including …