Essential design considerations for the resazurin reduction assay to noninvasively quantify cell expansion within perfused extracellular matrix scaffolds

JS Uzarski, MD DiVito, JA Wertheim, WM Miller - Biomaterials, 2017 - Elsevier
Precise measurement of cellularity within bioartificial tissues and extracellular matrix (ECM)
scaffolds is necessary to augment rigorous characterization of cellular behavior, as accurate …

Early osteogenic signal expression of rat bone marrow stromal cells is influenced by both hydroxyapatite nanoparticle content and initial cell seeding density in …

K Kim, D Dean, A Lu, AG Mikos, JP Fisher - Acta biomaterialia, 2011 - Elsevier
Incorporation of hydroxyapatite (HA) within a degradable polymeric scaffold may provide a
favorable synthetic microenvironment that more closely mimics natural bone tissue …

Cell seeding density is a critical determinant for copolymer scaffolds‐induced bone regeneration

MA Yassin, KN Leknes, TO Pedersen… - … Research Part A, 2015 - Wiley Online Library
Constructs intended for bone tissue engineering (TE) are influenced by the initial cell
seeding density. Therefore, the objective of this study was to determine the effect of bone …

Ex vivo non-invasive assessment of cell viability and proliferation in bio-engineered whole organ constructs

X Ren, LF Tapias, BJ Jank, DJ Mathisen, M Lanuti… - Biomaterials, 2015 - Elsevier
Decellularized organ scaffolds allow whole organ regeneration and study of cell behavior in
three-dimensional culture conditions. Cell viability within the bio-engineered organ …

[HTML][HTML] Unique animal friendly 3D culturing of human cancer and normal cells

AM Permlid, P Roci, E Fredlund, F Fält, E Önell… - Toxicology in Vitro, 2019 - Elsevier
Two-dimensional cell culturing has proven inadequate as a reliable preclinical tumour
model due to many inherent limitations. Hence, novel three-dimensional (3D) cell culture …

Effects of osteogenic differentiation inducers on in vitro expanded adult mesenchymal stromal cells

E Fiorentini, D Granchi, E Leonardi… - … Journal of Artificial …, 2011 - journals.sagepub.com
Purpose For bone regeneration therapy using stem cells, well-defined ex vivo protocols to
expand mesenchymal stromal cells (MSC), as well as assays to show their potential …

Noninvasive Real-Time Monitoring by AlamarBlue® During In Vitro Culture of Three-Dimensional Tissue-Engineered Bone Constructs

X Zhou, I Holsbeeks, S Impens, M Sonnaert… - … Engineering Part C …, 2013 - liebertpub.com
Bone tissue engineering (TE) aims to develop reproducible and predictive three-
dimensional (3D) TE constructs, defined as cell-seeded scaffolds produced by a controlled …

Osseointegration of zirconia and titanium implants in a rabbit tibiae model evaluated by microtomography, histomorphometry and fluorochrome labeling analyses

R Martins, TM Cestari, RVN Arantes… - Journal of …, 2018 - Wiley Online Library
Objectives This study compares the osseointegration of machined‐zirconia implants
containing yttria (M‐Y‐TZP) with machined (M‐Ti) and resorbable blast media (RBM‐Ti) …

Effect of cell‐seeded hydroxyapatite scaffolds on rabbit radius bone regeneration

CR Rathbone, T Guda, BM Singleton… - … Research Part A, 2014 - Wiley Online Library
Highly porous hydroxyapatite (HA) scaffolds were developed as bone graft substitutes using
a template coating process, characterized, and seeded with bone marrow‐derived …

Noninvasive real-time assessment of cell viability in a three-dimensional tissue

SH Mahfouzi, G Amoabediny, A Doryab… - … Engineering Part C …, 2018 - liebertpub.com
Maintaining cell viability within 3D tissue engineering scaffolds is an essential step toward a
functional tissue or organ. Assessment of cell viability in 3D scaffolds is necessary to control …