CRISPR/Cas and argonaute-based biosensors for pathogen detection

Z Yang, S Mao, L Wang, S Fu, Y Dong… - ACS …, 2023 - ACS Publications
Over the past few decades, pathogens have posed a threat to human security, and rapid
identification of pathogens should be one of the ideal methods to prevent major public …

[HTML][HTML] CRISPR-microfluidics nexus: Advancing biomedical applications for understanding and detection

ZS Razavi, M Soltani, H Pazoki-Toroudi… - Sensors and Actuators A …, 2024 - Elsevier
The integration of CRISPR technology with microfluidic-based biosensors has greatly
expanded its applications in medicine and molecular biology. This combination offers …

Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

J Qiao, Z Zhao, Y Li, M Lu, S Man, S Ye… - Critical Reviews in …, 2024 - Taylor & Francis
Food safety problems have become one of the most important public health issues
worldwide. Therefore, the development of rapid, effective and robust detection is of great …

CRISPR/Cas-powered amplification-free detection of nucleic acids: current state of the art, challenges, and futuristic perspectives

Y Li, Y Liu, X Tang, J Qiao, J Kou, S Man, L Zhu… - ACS …, 2023 - ACS Publications
CRISPR/Cas system is becoming an increasingly influential technology that has been
repositioned in nucleic acid detection. A preamplification step is usually required to improve …

A critical review of microfluidic systems for CRISPR assays

AS Avaro, JG Santiago - Lab on a Chip, 2023 - pubs.rsc.org
Reviewed are nucleic acid detection assays that incorporate clustered regularly interspaced
short palindromic repeats (CRISPR)-based diagnostics and microfluidic devices and …

Intelligent DNA-based Hydrogels: An Outstanding Programable and Biocompatible Material for Food Applications

G Li, H Huang, L Zou, X Zhang, X Lin, M Javed… - Trends in Food Science …, 2024 - Elsevier
Background Biocompatible smart materials have ushered in revolutionary applications,
conferring significant benefits upon humanity. Among these materials, DNA hydrogels stand …

CRISPR-based electrochemical biosensors: an alternative for point-of-care diagnostics?

DW Junior, LT Kubota - Talanta, 2024 - Elsevier
The combination of CRISPR technology and electrochemical sensors has sparked a
paradigm shift in the landscape of point-of-care (POC) diagnostics. This review explores the …

Machine Learning-Assisted, Dual-Channel CRISPR/Cas12a Biosensor-In-Microdroplet for Amplification-Free Nucleic Acid Detection for Food Authenticity Testing

Z Zhao, R Wang, X Yang, J Jia, Q Zhang, S Ye, S Man… - ACS …, 2024 - ACS Publications
The development of novel detection technology for meat species authenticity is imperative.
Here, we developed a machine learning-supported, dual-channel biosensor-in-microdroplet …

Fast and sensitive detection of viable Escherichia coli O157: H7 using a microwell-confined and propidium monoazide-assisted digital CRISPR microfluidic platform

W Yin, K Hu, B Yu, T Zhang, H Mei, B Zhang, Z Zou… - Lab on a Chip, 2024 - pubs.rsc.org
Escherichia coli O157: H7 is a major foodborne pathogen that poses a significant threat to
food safety and human health. Rapid and sensitive detection of viable Escherichia coli …

CRISPR for companion diagnostics in low-resource settings

Q Xu, Q Xu, C Lyon, TY Hu - Lab on a Chip, 2024 - pubs.rsc.org
New point-of-care tests (POCTs), which are especially useful in low-resource settings, are
needed to expand screening capacity for diseases that cause significant mortality …