Optical super-resolution microscopy unravels the molecular composition of functional protein complexes
MS Dietz, M Heilemann - Nanoscale, 2019 - pubs.rsc.org
Optical super-resolution microscopy has revolutionized our understanding of cell biology.
Next to visualizing cellular structures with near-molecular spatial resolution, an additional …
Next to visualizing cellular structures with near-molecular spatial resolution, an additional …
Circumvention of common labelling artefacts using secondary nanobodies
S Sograte-Idrissi, T Schlichthaerle, CJ Duque-Afonso… - Nanoscale, 2020 - pubs.rsc.org
A standard procedure to study cellular elements is via immunostaining followed by optical
imaging. This methodology typically requires target-specific primary antibodies (1. Abs) …
imaging. This methodology typically requires target-specific primary antibodies (1. Abs) …
PAINT using proteins: A new brush for super‐resolution artists
PAINT (points accumulation for imaging in nanoscale topography) refers to methods that
achieve the sparse temporal labeling required for super‐resolution imaging by using …
achieve the sparse temporal labeling required for super‐resolution imaging by using …
Self-limiting polymerization of DNA origami subunits with strain accumulation
Biology demonstrates how a near infinite array of complex systems and structures at many
scales can originate from the self-assembly of component parts on the nanoscale. But to fully …
scales can originate from the self-assembly of component parts on the nanoscale. But to fully …
The stability and number of nucleating interactions determine DNA hybridization rates in the absence of secondary structure
S Hertel, RE Spinney, SY Xu, TE Ouldridge… - Nucleic Acids …, 2022 - academic.oup.com
The kinetics of DNA hybridization are fundamental to biological processes and DNA-based
technologies. However, the precise physical mechanisms that determine why different DNA …
technologies. However, the precise physical mechanisms that determine why different DNA …
Binding of DNA origami to lipids: maximizing yield and switching via strand displacement
Liposomes are widely used as synthetic analogues of cell membranes and for drug delivery.
Lipid-binding DNA nanostructures can modify the shape, porosity and reactivity of …
Lipid-binding DNA nanostructures can modify the shape, porosity and reactivity of …
Design and synthesis of pleated DNA origami nanotubes with adjustable diameters
DNA origami allows for the synthesis of nanoscale structures and machines with nanometre
precision and high yields. Tubular DNA origami nanostructures are particularly useful …
precision and high yields. Tubular DNA origami nanostructures are particularly useful …
An update on molecular counting in fluorescence microscopy
Quantitative assessment of protein complexes, such as receptor clusters in the context of
cellular signalling, has become a pressing objective in cell biology. The advancements in …
cellular signalling, has become a pressing objective in cell biology. The advancements in …
Mechanisms underlying sequence-dependent DNA hybridisation rates in the absence of secondary structure
The kinetics of DNA hybridisation are fundamental to biological processes and DNA-based
technologies. However, the precise physical mechanisms that determine why different DNA …
technologies. However, the precise physical mechanisms that determine why different DNA …
Pushing the super-resolution limit: recent improvements in microscopy below the diffraction limit
Super-resolution microscopy has revolutionised the way we observe biological systems.
These methods are now a staple of fluorescence microscopy. Researchers have used super …
These methods are now a staple of fluorescence microscopy. Researchers have used super …