Understanding signaling and other complex biological processes requires elucidating the
critical roles of intrinsically disordered proteins (IDPs) and regions (IDRs), which represent∼ …

The hallmark of eukaryotic cells is the nucleus that contains the genome, enclosed by a
physical barrier known as the nuclear envelope (NE). On the one hand, this …

Lentiviruses can infect non-dividing cells, and various cellular transport proteins provide
crucial functions for lentiviral nuclear entry and integration. We previously showed that the …

Nuclear pore complexes (NPCs) are biological nanomachines that mediate the bidirectional
traffic of macromolecules between the cytoplasm and nucleus in eukaryotic cells. This …

The cytoplasmic side of eukaryotic cell membranes is covered by a dense actin rich cortex.
We present FCS and STED experiments showing that a dense membrane bound actin …

Retroviruses integrate their reverse transcribed genomes into host cell chromosomes as an
obligate step in virus replication. The nuclear envelope separates the chromosomes from …

Fluorescence correlation spectroscopy (FCS) is a widely used technique in biophysics and
has helped address many questions in the life sciences. It provides important advantages …

Many full-length proteins and protein regions lack stable tertiary and/or secondary structure
under physiological conditions in vitro. These proteins and regions, known as intrinsically …

We review some of the progress of single-molecule optical experiments in the past 20 years
and propose some perspectives for the coming years. We particularly focus on …

The nuclear pore complex (NPC) mediates the shuttle transport of macromolecules between
the nucleus and cytoplasm in eukaryotic cells. The permeability barrier formed by …