Transfection is a modern and powerful method used to insert foreign nucleic acids into
eukaryotic cells. The ability to modify host cells' genetic content enables the broad …

Extracellular vesicles (EVs) are naturally occurring cell-secreted nanoparticles that play
important roles in many physiological and pathological processes. EVs enable intercellular …

Ionizable lipid nanoparticles (LNPs) are the most clinically advanced nonviral platform for
mRNA delivery. While they have been explored for applications including vaccines and …

Conventional genome engineering with CRISPR–Cas9 creates double-strand breaks
(DSBs) that lead to undesirable byproducts and reduce product purity. Here we report an …

Chimeric antigen receptor (CAR) T cell therapy relies on the ex vivo manipulation of patient
T cells to create potent, cancer-targeting therapies, shown to be capable of inducing …

Lentiviral vectors (LVs) are potent tools for the delivery of genes of interest into mammalian
cells and are now commonly utilised within the growing field of cell and gene therapy for the …

Lipid‐based nanoparticles have emerged as a clinically viable platform technology to
deliver nucleic acids for a wide range of healthcare applications. Within this scope, one of …

Nucleic acid sensors, primarily TLR and RLR family members, as well as cGAS–STING
signalling, play a critical role in the preservation of cellular and organismal homeostasis …

Cancer is one of the primary causes of death worldwide, and its morbidity and mortality rates
are increasing rapidly. However, standard treatment modalities (surgery, radiotherapy …

Lipid-based carriers represent the most widely used alternative to viral vectors for gene
expression and gene silencing purposes. This class of non-viral vectors is particularly …